Fish Interleukin 6, IL-6 ELISA Kit | IL-6 elisa kit

Fish Interleukin 6, IL-6 ELISA Kit

Cat. Num. AAA702353

• Gene Names: Il6; ILg6; Ifnb2
• Reactivity: Fish
• Synonyms: Interleukin 6; IL-6; Fish Interleukin 6; IL-6 ELISA Kit; IL-6 elisa kit

• Reactivity: Fish
• Specificity: This assay has high sensitivity and excellent specificity for detection of fish IL-6. No significant cross-reactivity or interference between fish IL-6 and analogues was observed.
• Sequence Length: 211

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 12.5 pg/ml -200 pg/mL
• Sensitivity: <12.5 pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-6 elisa kit

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-6. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated IL-6. A competitive inhibition reaction is launched between IL-6 (Standards or samples) and Biotin-conjugated IL-6 with the pre-coated antibody specific for IL-6. The more amount of IL-6 in samples, the less antibody bound by Biotin-conjugated IL-6. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of IL-6 in the sample. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 7549769
• NCBI GeneID: 24498
• NCBI Accession #: NP_036721.1
• NCBI GenBank Nucleotide #: NM_012589.2
• UniProt Accession #: P20607
• Molecular Weight: 24,357 Da
• NCBI Official Full Name: interleukin-6
• NCBI Official Synonym Full Names: interleukin 6
• NCBI Official Symbol: Il6
• NCBI Official Synonym Symbols: ILg6; Ifnb2
• NCBI Protein Information: interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)
• UniProt Protein Name: Interleukin-6
• UniProt Gene Name: Il6
• UniProt Synonym Gene Names: Il-6; IL-6
• UniProt Entry Name: IL6_RAT

NCBI Description

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

Uniprot Description

Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation