Typical Testing Data/Standard Curve (for reference only)
Bovine Interleukin 6 ELISA Kit | IL-6 elisa kit
Bovine Interleukin 6 ELISA Kit
Gene Names
Il6; ILg6; Ifnb2
Reactivity
Bovine
Synonyms
Interleukin 6; Bovine Interleukin 6 ELISA Kit; IL-6 elisa kit
Reactivity
Bovine
Specificity
This assay has high sensitivity and excellent specificity for detection of IL-6. No significant cross-reactivity or interference between IL-6 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between IL-6 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IL-6 elisa kit
Intended Uses: This IL-6 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine IL-6. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: IL-6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-6 antibody and an IL-6-HRP conjugate. The assay sample and buffer are incubated together with IL-6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-6 concentration since IL-6 from samples and IL-6-HRP conjugate compete for the anti-IL-6 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-6 from the sample, fewer sites are left to bind IL-6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-6 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: IL-6 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-IL-6 antibody and an IL-6-HRP conjugate. The assay sample and buffer are incubated together with IL-6-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-6 concentration since IL-6 from samples and IL-6-HRP conjugate compete for the anti-IL-6 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-6 from the sample, fewer sites are left to bind IL-6-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-6 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for IL-6 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,357 Da
NCBI Official Full Name
interleukin-6
NCBI Official Synonym Full Names
interleukin 6
NCBI Official Symbol
Il6
NCBI Official Synonym Symbols
ILg6; Ifnb2
NCBI Protein Information
interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)
UniProt Protein Name
Interleukin-6
UniProt Gene Name
Il6
UniProt Synonym Gene Names
Il-6; IL-6
UniProt Entry Name
IL6_RAT
NCBI Description
a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]
Uniprot Description
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().
Research Articles on IL-6
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Product Notes
The Bovine IL-6 il6 (Catalog #AAA733925) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA733925 ELISA Kit recognizes Bovine IL-6. It is sometimes possible for the material contained within the vial of "Interleukin 6, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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