Sheep IL-6 ELISA Kit | IL-6 elisa kit

Sheep IL-6 (Interleukin 6) ELISA Kit

Cat. Num. AAA2500510

• Gene Names: Il6; ILg6; Ifnb2
• Reactivity: Sheep
• Synonyms: IL-6; Sheep IL-6 (Interleukin 6) ELISA Kit; IL-6 elisa kit

• Reactivity: Sheep
• Specificity: This kit recognizes Sheep IL-6 in samples. No significant cross-reactivity or interference between Sheep IL-6 and analogues was observed.
• Sequence Length: 211

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: 46.875pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Sheep IL-6 were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Sheep IL-6 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-6 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Sheep IL-6 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Sheep IL-6. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Sheep IL-6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Sheep IL-6, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Sheep IL-6. You can calculate the concentration of Sheep IL-6 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7549769
• NCBI GeneID: 24498
• NCBI Accession #: NP_036721.1
• NCBI GenBank Nucleotide #: NM_012589.2
• UniProt Accession #: P20607
• Molecular Weight: 24,357 Da
• NCBI Official Full Name: interleukin-6
• NCBI Official Synonym Full Names: interleukin 6
• NCBI Official Symbol: Il6
• NCBI Official Synonym Symbols: ILg6; Ifnb2
• NCBI Protein Information: interleukin-6; IL-6; Interleukin 6 (interferon, beta 2)
• UniProt Protein Name: Interleukin-6
• UniProt Gene Name: Il6
• UniProt Synonym Gene Names: Il-6; IL-6
• UniProt Entry Name: IL6_RAT

NCBI Description

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

Uniprot Description

Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation ().