IgG1 Recombinant Protein | IGHG1 recombinant protein

IgG1 Protein, Human, Recombinant (103 Cys/Ser), Biotinylated

Cat. Num. AAA8120440

• Purity: >90% as determined by SDS-PAGE
• Synonyms: IgG1; IgG1 Protein; Human; Recombinant (103 Cys/Ser); Biotinylated; Human IgG1-Fc Protein (103 Cys/Ser); IgG1 Fc Protein; Ighg1 Protein; IGHG1 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile Sterile PBS. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Glu99-Lys330

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Glu
• Tag: Native
• Protein Construction: A DNA sequence encoding the IGHG1 (AAC82527.1)(Glu99-Lys330)(one aa mutation,103 Cys/Ser) was expressed. The purified protein was biotinylated in vitro.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for IGHG1 recombinant protein

Background: As a monomeric immunoglobulin that is predominately involved in the secondary antibody response and the only isotype that can pass through the human placenta, Immunoglobulin G (IgG) is synthesized and secreted by plasma B cells, and constitutes 75% of serum immunoglobulins in humans. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as antibody-dependent cell-mediated cytotoxicity (ADCC). IgG tetramer contains two heavy chains (5kDa) and two light chains (25kDa) linked by disulfide bonds, that is the two identical halves form the Y-like shape. IgG is digested by pepsin proteolysis into Fab fragment (antigen-binding fragment) and Fc fragment ("crystallizable" fragment). IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors (FcgammaR) on phagocytic cells with high affinity. Fc fragment is demonstrated to mediate phagocytosis, trigger inflammation, and target Ig to particular tissues. Protein G or Protein A on the surface of certain Staphylococcal and Streptococcal strains specifically binds with the Fc region of IgGs, and has numerous applications in biotechnology as a reagent for affinity purification. Recombinant IgG Fc Region is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases.

References

Carosella ED. et al., 1988, Cell Immunol. 112: 262-70. Eckle I. et al., 1990, Clin Exp Immunol. 81: 352-6. Lorriaine C. et al., 1995, J Biol Chem. 270:8164-71. Gomez-Guerrero C. et al., 2000, J Immunol. 164: 2092-101. Sprague ER. et al., 2004, J Biol Chem. 14: 14184-93. Anthony RM. et al., 2008, Science. 320: 373-6.

NCBI and Uniprot Product Information

• NCBI GI #: 184747
• Molecular Weight: 36,106 Da
• NCBI Official Full Name: immunoglobulin gamma-1 heavy chain constant region, partial
• Protein Family: Ig gamma-1 chain C region