Western Blot (WB)
(Anti-HSPB2 Picoband antibody, MBS177835, Western blottingAll lanes: Anti HSPB2 (MBS177835) at 0.5ug/mlLane 1: Rat Cardiac Muscle Tissue Lysate at 50ugLane 2: Rat Skeletal Muscle Tissue Lysate at 50ugPredicted bind size: 20KDObserved bind size: 20KD )
HSPB2 Polyclonal Antibody | anti-HSPB2 antibody
Anti-HSPB2 Antibody
Gene Names
HSPB2; MKBP; HSP27; Hs.78846; LOH11CR1K
Reactivity
RatPredicted to work with: Human
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
HSPB2; Polyclonal Antibody; Anti-HSPB2 Antibody; Heat shock protein beta-2; DMPK-binding protein; Heat shock 27kD protein 2; Heat shock 27kDa protein 2; Heat-shock protein beta-2; Hs.78846; HSP27; HspB2; HSPB2_HUMAN; LOH11CR1K; MGC133245; MKBP; heat shock 27kDa protein 2(HspB2); anti-HSPB2 antibody
Reactivity
Rat
Predicted to work with: Human
Predicted to work with: Human
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
182
Applicable Applications for anti-HSPB2 antibody
Western Blot (WB)
Application Notes
Western Blot Concentration: 0.1-0.5ug/ml
Immunogen
E Coli-derived human HSPB2 recombinant protein (Position: M1-P182). Human HSPB2 shares 97.3% amino acid (aa) sequence identity with both mouse and rat HSPB2.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-HSPB2 Picoband antibody, MBS177835, Western blottingAll lanes: Anti HSPB2 (MBS177835) at 0.5ug/mlLane 1: Rat Cardiac Muscle Tissue Lysate at 50ugLane 2: Rat Skeletal Muscle Tissue Lysate at 50ugPredicted bind size: 20KDObserved bind size: 20KD )
Western Blot (WB)
(Anti-HSPB2 Picoband antibody, MBS177835, Western blottingAll lanes: Anti HSPB2 (MBS177835) at 0.5ug/mlLane 1: Rat Cardiac Muscle Tissue Lysate at 50ugLane 2: Rat Skeletal Muscle Tissue Lysate at 50ugPredicted bind size: 20KDObserved bind size: 20KD )
Related Product Information for anti-HSPB2 antibody
Description: Rabbit IgG polyclonal antibody for Heat shock protein beta-2(HSPB2) detection. Tested with WB in Human;Rat.
Background: Alpha-B-crystallin is a member of the alpha-crystallin/small heat-shock protein (HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al. (1997) identified a gene transcribed in the opposite direction. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They termed the gene HSPB2. Iwaki et al. (1997) also determined the complete genomic structure of the rat homolog of HSPB2. Southern blot analysis demonstrated that HSPB2 is expressed preferentially in skeletal muscle and heart but not in the lens, while the neighboring CRYAB gene is highly expressed in all 3 tissues. The 2 related genes are arranged in a head-to-head manner with an intergenic sequence of less than 1 kb, raising a possibility of shared regulatory elements for their expression.
Background: Alpha-B-crystallin is a member of the alpha-crystallin/small heat-shock protein (HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al. (1997) identified a gene transcribed in the opposite direction. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They termed the gene HSPB2. Iwaki et al. (1997) also determined the complete genomic structure of the rat homolog of HSPB2. Southern blot analysis demonstrated that HSPB2 is expressed preferentially in skeletal muscle and heart but not in the lens, while the neighboring CRYAB gene is highly expressed in all 3 tissues. The 2 related genes are arranged in a head-to-head manner with an intergenic sequence of less than 1 kb, raising a possibility of shared regulatory elements for their expression.
References
1. Iwaki, A., Nagano, T., Nakagawa, M., Iwaki, T., Fukumaki, Y. Identification and characterization of the gene encoding a new member of the alpha-crystallin/Small hsp family, closely linked to the alpha-B-crystallin gene in a head-to-head manner. Genomics 45: 386-394, 1997.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,233 Da
NCBI Official Full Name
heat shock protein beta-2
NCBI Official Synonym Full Names
heat shock protein family B (small) member 2
NCBI Official Symbol
HSPB2
NCBI Official Synonym Symbols
MKBP; HSP27; Hs.78846; LOH11CR1K
NCBI Protein Information
heat shock protein beta-2
UniProt Protein Name
Heat shock protein beta-2
Protein Family
UniProt Gene Name
HSPB2
UniProt Synonym Gene Names
HspB2; MKBP
UniProt Entry Name
HSPB2_HUMAN
NCBI Description
The protein encoded by this gene belongs to the superfamily of small heat-shock proteins containing a conservative alpha-crystallin domain at the C-terminal part of the molecule. The protein is expressed preferentially in the heart and skeletal muscle. This protein regulates Myotonic Dystrophy Protein Kinase, which plays an important role in maintenance of muscle structure and function. [provided by RefSeq, Dec 2012]
Uniprot Description
HSPB2: May regulate the kinase DMPK. Belongs to the small heat shock protein (HSP20) family.
Protein type: Chaperone; Heat shock protein
Chromosomal Location of Human Ortholog: 11q22-q23
Cellular Component: cytoplasm; cytosol; nucleus
Molecular Function: enzyme activator activity; protein binding
Biological Process: positive regulation of catalytic activity; response to unfolded protein
Research Articles on HSPB2
Similar Products
Product Notes
The HSPB2 hspb2 (Catalog #AAA177835) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-HSPB2 Antibody reacts with Rat Predicted to work with: Human and may cross-react with other species as described in the data sheet. AAA Biotech's HSPB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the HSPB2 hspb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSPB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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