Rat Glutamate Cysteine Ligase, Modifier Subunit (GCLM) ELISA Kit | GCLM elisa kit

Rat Glutamate Cysteine Ligase, Modifier Subunit (GCLM) ELISA Kit

Cat. Num. AAA2022418

• Gene Names: GCLM; GLCLR
• Reactivity: Rat
• Synonyms: Glutamate Cysteine Ligase; Modifier Subunit (GCLM); Rat Glutamate Cysteine Ligase; Modifier Subunit (GCLM) ELISA Kit; GLCLR; GECS; GCL; Gamma Glutamylcysteine Synthetase; Glutamate Cysteine Ligase Regulatory; Gamma-ECS regulatory subunit; Glutamate cysteine ligase modifier subunit; GCLM elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Glutamate Cysteine Ligase, Modifier Subunit (GCLM).; No significant cross-reactivity or interference between Glutamate Cysteine Ligase, Modifier Subunit (GCLM) and analogues was observed.
• Sequence Length: 252

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.052ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glutamate Cysteine Ligase, Modifier Subunit (GCLM) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glutamate Cysteine Ligase, Modifier Subunit (GCLM) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GCLM elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutamate Cysteine Ligase, Modifier Subunit (GCLM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutamate Cysteine Ligase, Modifier Subunit (GCLM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutamate Cysteine Ligase, Modifier Subunit (GCLM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glutamate Cysteine Ligase, Modifier Subunit (GCLM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for GCLM elisa kit

Enzyme & Kinase; Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 27469699
• NCBI GeneID: 2730
• UniProt Accession #: P48507; Q6FHC1; Q9NPX9; Q9NU74; A8K334; D3DT45; M5A959
• Molecular Weight: 28,135 Da
• NCBI Official Full Name: Glutamate-cysteine ligase, modifier subunit
• NCBI Official Synonym Full Names: glutamate-cysteine ligase, modifier subunit
• NCBI Official Symbol: GCLM
• NCBI Official Synonym Symbols: GLCLR
• NCBI Protein Information: glutamate--cysteine ligase regulatory subunit
• UniProt Protein Name: Glutamate--cysteine ligase regulatory subunit
• Protein Family: Glutamate--cysteine ligase regulatory
• UniProt Gene Name: GCLM
• UniProt Synonym Gene Names: GLCLR
• UniProt Entry Name: GSH0_HUMAN

NCBI Description

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]

Uniprot Description

GCLM: Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. [provided by RefSeq, Jul 2008]

Protein type: Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 1p22.1

Cellular Component: glutamate-cysteine ligase complex; cytosol

Molecular Function: protein heterodimerization activity; glutamate-cysteine ligase activity; enzyme regulator activity; glutamate-cysteine ligase catalytic subunit binding

Biological Process: response to drug; glutamate metabolic process; response to nitrosative stress; apoptotic mitochondrial changes; cysteine metabolic process; sulfur amino acid metabolic process; xenobiotic metabolic process; glutathione biosynthetic process; negative regulation of neuron apoptosis; response to oxidative stress; regulation of mitochondrial depolarization; regulation of blood vessel size; positive regulation of glutamate-cysteine ligase activity

Disease: Myocardial Infarction, Susceptibility To