Human Insulin-like growth factors 2 ELISA Kit | IGF-2 elisa kit
Human Insulin-like growth factors 2 (IGF-2) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate IGF-2 in Human serum, plasma. An antibody specific for IGF-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IGF-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IGF-2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IGF-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]
Uniprot Description
IGF2: The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SRS). A clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations. The phenotypic expression changes during childhood and adolescence, with the facial features and asymmetry usually becoming more subtle with age. Belongs to the insulin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: extracellular region; plasma membrane
Molecular Function: growth factor activity; hormone activity; insulin receptor binding; insulin-like growth factor receptor binding; protein binding; protein serine/threonine kinase activator activity; receptor activator activity
Biological Process: cellular protein metabolic process; embryonic placenta development; genetic imprinting; glucose metabolic process; in utero embryonic development; insulin receptor signaling pathway; multicellular organism development; negative regulation of transcription from RNA polymerase II promoter; ossification; platelet degranulation; positive regulation of activated T cell proliferation; positive regulation of catalytic activity; positive regulation of cell division; positive regulation of cell proliferation; positive regulation of glycogen biosynthetic process; positive regulation of insulin receptor signaling pathway; positive regulation of MAPK cascade; positive regulation of mitotic nuclear division; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase B signaling; positive regulation of protein phosphorylation; regulation of histone modification; regulation of muscle cell differentiation; regulation of transcription, DNA-templated; skeletal system development
Disease: Beckwith-wiedemann Syndrome; Growth Restriction, Severe, With Distinctive Facies; Silver-russell Syndrome; Wilms Tumor 1
Research Articles on IGF-2
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Product Notes
The Human IGF-2 igf2 (Catalog #AAA285701) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA285701 ELISA Kit recognizes Human IGF-2. It is sometimes possible for the material contained within the vial of "Insulin-like growth factors 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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