ICAM-5 recombinant protein

Recombinant human ICAM-5 protein

Cat. Num. AAA201270

• Gene Names: ICAM5; TLN; TLCN
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: ICAM-5; Recombinant human ICAM-5 protein; Intercellular adhesion molecule 5; ICAM5; Telencephalin; telencephalin; TLN; TLCN; TLNtelencephalin; TLCNTelencephalin; ICAM-5 recombinant protein

• Host: HEK293
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
• Concentration: 0.5mg/ml (determined by Bradford assay) (varies by lot)
• Sequence Positions: 23-835aa
• Sequence: LFGLSAVSQE PFWADLQPRV AFVERGGSLW LNCSTNCPRP ERGGLETSLR RNGTQRGLRW LARQLVDIRE PETQPVCFFR CARRTLQARG LIRTFQRPDR VELMPLPPWQ PVGENFTLSC RVPGAGPRAS LTLTLLRGAQ ELIRRSFAGE PPRARGAVLT ATVLARREDH GANFSCRAEL DLRPHGLGLF ENSSAPRELR TFSLSPDAPR LAAPRLLEVG SERPVSCTLD GLFPASEARV YLALGDQNLS PDVTLEGDAF VATATATASA EQEGARQLVC NVTLGGENRE TRENVTIYSF PAPLLTLSEP SVSEGQMVTV TCAAGAQALV TLEGVPAAVP GQPAQLQLNA TENDDRRSFF CDATLDVDGE TLIKNRSAEL RVLYAPRLDD SDCPRSWTWP EGPEQTLRCE ARGNPEPSVH CARSDGGAVL ALGLLGPVTR ALSGTYRCKA ANDQGEAVKD VTLTVEYAPA LDSVGCPERI TWLEGTEASL SCVAHGVPPP DVICVRSGEL GAVIEGLLRV AREHAGTYRC EATNPRGSAA KNVAVTVEYG PRFEEPSCPS NWTWVEGSGR LFSCEVDGKP QPSVKCVGSG GATEGVLLPL APPDPSPRAP RIPRVLAPGI YVCNATNRHG SVAKTVVVSA ESPPEMDEST CPSHQTWLEG AEASALACAA RGRPSPGVRC SREGIPWPEQ QRVSREDAGT YHCVATNAHG TDSRTVTVGV EYRPVVAELA ASPPGGVRPG GNFTLTCRAE AWPPAQISWR APPGALNIGL SSNNSTLSVA GAMGSHGGEY ECAATNAHGR HARRITVRVA GPW

• Applicable Applications for ICAM-5 recombinant protein: SDS-PAGE
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: hIgG-His-Tag
• Predicted N-term: Leu23
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for ICAM-5 recombinant protein

ICAM-5, also known as telencephalin, is a cell surface glycoprotein belonging to the immunoglobulin superfamily. Human ICAM-5 is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. The tissue of ICAM-5, being expressed only in telencephalic regions of the central nervous system. Like other ICAMs, ICAM-5 binds to the leukocyte integrin LFA-1. Recombinant human ICAM-5, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Categories/Family for ICAM-5 recombinant protein

Immunology; Adhesion & Movement; Neuroscience; Proteins

References

Yoshihara, Y. et al. (1994) Neuron 12:541-553. Tain, L. et al. (2000) Eur. J. Immunol. 30:810-818. Mizuno, T. et al. (1997) J. Biol. Chem. 272:1156-1163.

NCBI and Uniprot Product Information

• NCBI GI #: 189571583
• NCBI GeneID: 7087
• NCBI Accession #: NP_003250
• NCBI GenBank Nucleotide #: NM_003259
• UniProt Accession #: Q9UMF0
• Molecular Weight: 95kDa
• NCBI Official Full Name: intercellular adhesion molecule 5
• NCBI Official Synonym Full Names: intercellular adhesion molecule 5
• NCBI Official Symbol: ICAM5
• NCBI Official Synonym Symbols: TLN; TLCN
• NCBI Protein Information: intercellular adhesion molecule 5
• UniProt Protein Name: Intercellular adhesion molecule 5
• UniProt Gene Name: ICAM5
• UniProt Synonym Gene Names: TLCN; TLN; ICAM-5
• UniProt Entry Name: ICAM5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the intercellular adhesion molecule (ICAM) family. All ICAM proteins are type I transmembrane glycoproteins, contain 2-9 immunoglobulin-like C2-type domains, and bind to the leukocyte adhesion LFA-1 protein. This protein is expressed on the surface of telencephalic neurons and displays two types of adhesion activity, homophilic binding between neurons and heterophilic binding between neurons and leukocytes. It may be a critical component in neuron-microglial cell interactions in the course of normal development or as part of neurodegenerative diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

ICAM5: ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). Belongs to the immunoglobulin superfamily. ICAM family.

Protein type: Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: integrin binding; protein binding

Biological Process: cell-cell adhesion; cell adhesion; phagocytosis