Human Perilipin 1 (PLIN1) RTU ELISA Kit | PLIN1 rtu elisa kit

Human Perilipin 1 (PLIN1) ELISA Kit

Cat. Num. AAA4501919

• Gene Names: PLIN1; PERI; PLIN; FPLD4
• Reactivity: Human
• Synonyms: Perilipin 1 (PLIN1); Human Perilipin 1 (PLIN1) ELISA Kit; PERI; PLIN1; Lipid Droplet Associated Protein; PLIN1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 522

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.053ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PLIN1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLIN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PLIN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLIN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PLIN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 223718203
• NCBI GeneID: 5346
• NCBI Accession #: NP_001138783.1
• NCBI GenBank Nucleotide #: NM_001145311.1
• UniProt Accession #: O60240; Q8N5Y6
• Molecular Weight: 55,990 Da
• NCBI Official Full Name: perilipin-1
• NCBI Official Synonym Full Names: perilipin 1
• NCBI Official Symbol: PLIN1
• NCBI Official Synonym Symbols: PERI; PLIN; FPLD4
• NCBI Protein Information: perilipin-1
• UniProt Protein Name: Perilipin-1
• Protein Family: Perilipin
• UniProt Gene Name: PLIN1
• UniProt Synonym Gene Names: PERI; PLIN

NCBI Description

The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Feb 2009]

Uniprot Description

Modulator of adipocyte lipid metabolism. Coats lipid storage droplets to protect them from breakdown by hormone-sensitive lipase (HSL). Its absence may result in leanness. Plays a role in unilocular lipid droplet formation by activating CIDEC. Their interaction promotes lipid droplet enlargement and directional net neutral lipid transfer. May modulate lipolysis and triglyceride levels.