Typical Testing Data/Standard Curve (for reference only)
(Mouse CD48 PicoKine ELISA Kit standard curve )
Mouse CD48 ELISA Kit | CD48 elisa kit
Mouse CD48 PicoKine ELISA Kit
Gene Names
Cd48; BCM1; BLAST; Bcm-1; BLAST1; SLAMF2; Sgp-60; BLAST-1; MEM-102; AI449234; AW610730
Reactivity
Mouse
Synonyms
CD48; Mouse CD48 PicoKine ELISA Kit; CD48 antigen; CD48 molecule; Antigen CD48; B cell membrane protein; B lymphocyte activation marker BLAST 1 [Precursor]; B lymphocyte activation marker BLAST 1; B-cell activation marker; B-lymphocyte activation marker BLAST-1; BCM 1 surface antigen; BCM1; BCM1 surface antigen; BLAST 1; BLAST; BLAST1; CD 48; CD48 antigen (B cell membrane protein); CD48 protein; CD48_HUMAN; hCD48; Leucocyte antigen MEM 102; Leukocyte antigen MEM-102; mCD48; MEM 102; MEM-102; MEM102; Signaling lymphocytic activation molecule 2; SLAM family member 2; SLAMF 2; SLAMF2; TCT.1; CD48 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse CD48
Sequence Length
240
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
62.5pg/ml-4000pg/ml
Sensitivity
< 10pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Mouse CD48 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse CD48 PicoKine ELISA Kit standard curve )
Related Product Information for CD48 elisa kit
Principle of the assay: mouse CD48 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD48 has been precoated onto 96-well plates. Standards(NSO, F23-S217) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD48 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD48 amount of sample captured in plate.
Background: CD48 antigen(Cluster of Differentiation 48), also known as BLAST-1 or SLAMF2, is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily(IgSF) which includes SLAM(signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. It is mapped to 1q23.3. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor.
Background: CD48 antigen(Cluster of Differentiation 48), also known as BLAST-1 or SLAMF2, is a protein that in humans is encoded by the CD48 gene. CD48 is a member of the CD2 subfamily of the immunoglobulin superfamily(IgSF) which includes SLAM(signaling lymphocyte activation molecules) proteins, such as CD84, CD150, CD229 and CD244. It is mapped to 1q23.3. CD48 is found on the surface of lymphocytes and other immune cells, dendritic cells and endothelial cells, and participates in activation and differentiation pathways in these cells. The encoded protein does not have a transmembrane domain, however, but is held at the cell surface by a GPI anchor via a C-terminal domain which maybe cleaved to yield a soluble form of the receptor.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
27,383 Da
NCBI Official Full Name
CD48 antigen
NCBI Official Synonym Full Names
CD48 antigen
NCBI Official Symbol
Cd48
NCBI Official Synonym Symbols
BCM1; BLAST; Bcm-1; BLAST1; SLAMF2; Sgp-60; BLAST-1; MEM-102; AI449234; AW610730
NCBI Protein Information
CD48 antigen; HM48-1; BCM1 surface antigen; SLAM family member 2; MRC OX-45 surface antigen; signaling lymphocytic activation molecule 2
UniProt Protein Name
CD48 antigen
Protein Family
UniProt Gene Name
Cd48
UniProt Synonym Gene Names
Bcm-1; SLAMF2
UniProt Entry Name
CD48_MOUSE
Uniprot Description
CD48: Ligand for CD2. Might facilitate interaction between activated lymphocytes. Probably involved in regulating T-cell activation.
Protein type: Membrane protein, GPI anchor
Cellular Component: protein complex; anchored to plasma membrane; membrane; plasma membrane; integral to membrane; lipid raft; external side of plasma membrane
Molecular Function: protein binding; receptor activity; antigen binding
Biological Process: T cell activation; signal transduction; mast cell activation
Research Articles on CD48
Similar Products
Product Notes
The Mouse CD48 cd48 (Catalog #AAA177229) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177229 ELISA Kit recognizes Mouse CD48. It is sometimes possible for the material contained within the vial of "CD48, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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