Histone H1.1 Recombinant Protein | Hist1h1a recombinant protein

Recombinant Mouse Histone H1.1(Hist1h1a)

Cat. Num. AAA952901

• Gene Names: Hist1h1a; H1a; H1.1; H1f1; H1var3
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Histone H1.1; Recombinant Mouse Histone H1.1(Hist1h1a); H1 VAR.3; Histone H1a; H1a; Hist1h1a recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2-213aa; Full Length of Mature Protein
• Sequence: SETAPVAQAASTATEKPAAAKKTKKPAKAAAPRKKPAGPSVSELIVQAVSSSKERSGVSLAALKKSLAAAGYDVEKNNSRIKLGLKSLVNKGTLVQTKGTGAAGSFKLNKKAESKAITTKVSVKAKASGAAKKPKKTAGAAAKKTVKTPKKPKKPAVSKKTSKSPKKPKVVKAKKVAKSPAKAKAVKPKASKAKVTKPKTPAKPKKAAPKKK

• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

Related Product Information for Hist1h1a recombinant protein

Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation.

References

Isolation and characterization of two replication-dependent mouse H1 histone genes.Dong Y., Sirotkin A.M., Yang Y.-S., Brown D.T., Sittman D.B., Skoultchi A.I.Nucleic Acids Res. 22:1421-1428(1994) Expression of murine H1 histone genes during postnatal development.Franke K., Drabent B., Doenecke D.Biochim. Biophys. Acta 1398:232-242(1998) The human and mouse replication-dependent histone genes.Marzluff W.F., Gongidi P., Woods K.R., Jin J., Maltais L.J.Genomics 80:487-498(2002) Lineage-specific biology revealed by a finished genome assembly of the mouse.Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.PLoS Biol. 7:E1000112-E1000112(2009) Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. In vivo phosphorylation of histone H1 variants during the cell cycle.Talasz H., Helliger W., Puschendorf B., Lindner H.Biochemistry 35:1761-1767(1996) Spermatogenesis in mice is not affected by histone H1.1 deficiency.Rabini S., Franke K., Saftig P., Bode C., Doenecke D., Drabent B.Exp. Cell Res. 255:114-124(2000) SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways.Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.Mol. Cell 50:919-930(2013) Citrullination regulates pluripotency and histone H1 binding to chromatin.Christophorou M.A., Castelo-Branco G., Halley-Stott R.P., Oliveira C.S., Loos R., Radzisheuskaya A., Mowen K.A., Bertone P., Silva J.C., Zernicka-Goetz M., Nielsen M.L., Gurdon J.B., Kouzarides T.Nature 507:104-108(2014)

NCBI and Uniprot Product Information

• NCBI GI #: 21426823
• NCBI GeneID: 80838
• NCBI Accession #: NP_085112.1
• NCBI GenBank Nucleotide #: NM_030609.3
• UniProt Accession #: P43275; Q5SZ98
• Molecular Weight: 48.7 kDa
• NCBI Official Full Name: histone H1.1
• NCBI Official Synonym Full Names: histone cluster 1, H1a
• NCBI Official Symbol: Hist1h1a
• NCBI Official Synonym Symbols: H1a; H1.1; H1f1; H1var3
• NCBI Protein Information: histone H1.1
• UniProt Protein Name: Histone H1.1
• Protein Family: Histone
• UniProt Gene Name: Hist1h1a
• UniProt Synonym Gene Names: H1a; H1f1; H1a
• UniProt Entry Name: H11_MOUSE

NCBI Description

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. [provided by RefSeq, Aug 2015]

Uniprot Description

H1F1: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation. Belongs to the histone H1/H5 family.

Protein type: DNA-binding

Cellular Component: chromosome; nucleosome; nucleus

Molecular Function: DNA binding; protein binding

Biological Process: nucleosome assembly; spermatogenesis