Pig 5'-AMP-activated protein kinase catalytic subunit alpha-2 ELISA Kit | PRKAA2 elisa kit

Pig 5'-AMP-activated protein kinase catalytic subunit alpha-2 ELISA Kit

Cat. Num. AAA288303

• Gene Names: PRKAA2; AMPKA2
• Reactivity: Pig
• Synonyms: 5'-AMP-activated protein kinase catalytic subunit alpha-2; Pig 5'-AMP-activated protein kinase catalytic subunit alpha-2 ELISA Kit; PRKAA2 elisa kit

• Reactivity: Pig
• Specificity: This assay recognizes recombinant and natural pig AMPK subunit alpha-2. No significant cross-reactivity or interference was observed.
• Sequence Length: 552

• Samples: Serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Detection Range: 78.0 pg/ml -5000 pg/ml.
• Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of pig AMPK subunit alpha-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PRKAA2 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AMPK subunit alpha-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AMPK subunit alpha-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AMPK subunit alpha-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AMPK subunit alpha-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 47523598
• NCBI GeneID: 397504
• NCBI Accession #: NP_999431.1
• NCBI GenBank Nucleotide #: NM_214266.1
• UniProt Accession #: Q28948; Q7YRX9
• Molecular Weight: 62,325 Da
• NCBI Official Full Name: 5'-AMP-activated protein kinase catalytic subunit alpha-2
• NCBI Official Symbol: PRKAA2
• NCBI Official Synonym Symbols: AMPKA2
• NCBI Protein Information: 5'-AMP-activated protein kinase catalytic subunit alpha-2
• UniProt Protein Name: 5'-AMP-activated protein kinase catalytic subunit alpha-2
• Protein Family: 5'-AMP-activated protein kinase
• UniProt Gene Name: PRKAA2
• UniProt Synonym Gene Names: AMPK2; AMPK subunit alpha-2; ACACA kinase; HMGCR kinase
• UniProt Entry Name: AAPK2_PIG

Uniprot Description

AMPKA2: a catalytic subunit of AMP-activated protein kinase (AMPK). Acts as an energy sensor, playing a key role in regulating cellular energy metabolism. A protein kinase of the CAMKL family whose activation is regulated by the balance between ADP/AMP/ATP, and intracellular Ca(2+) levels. Acts as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-ADP and -AMP rise in response to fuel limitation and/or hypoxia. Activates energy-producing pathways and inhibits energy-consuming processes. Restores ATP levels in cells by switching off anabolic and switching on catabolic pathways. Activated primarily by rising ADP levels and not, as previously thought, solely by AMP. AMPK resembles an adenylate charge regulatory system in which anabolic and catabolic pathways are regulated by adenine nucleotide ratios. Acts via direct phosphorylation of metabolic enzymes and transcription regulators. Regulates fatty acid synthesis by phosphorylating acetyl-CoA carboxylase. Regulates cholesterol synthesis by phosphorylating and inactivating hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Activated by at least two distinct upstream kinases: the tumor suppressor LKB1 and CaMKK2. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. AMPK is a heterotrimer of an alpha catalytic subunit (AMPKA1 or -2), a beta (AMPKB1 or -2) and a gamma non-catalytic subunit (AMPKG1, -2 or -3). Different possible combinations of subunits give rise to 12 different holoenzymes. Binding of ADP or AMP to non-catalytic gamma subunit (PRKAG1, -2 or -3) results in allosteric activation. AMPK is activated by antihyperglycemic drug metformin, a drug prescribed to patients with type 2 diabetes: in vivo, metformin seems to mainly inhibit liver gluconeogenesis. However, metformin can be used to activate AMPK in muscle and other cells in culture or ex vivo. Selectively inhibited by compound C (6-[4-(2-Piperidin-1-yl-ethoxy)-phenyl)]-3-pyridin-4-yl-pyyrazolo[1,5-a] pyrimidine. Activated by resveratrol, a natural polyphenol present in red wine, and S17834, a synthetic polyphenol. Salicylate/aspirin directly activates kinase activity. Studies in the mouse suggest that AMPK2 may control whole-body insulin sensitivity and is necessary for maintaining myocardial energy homeostasis during ischemia.

Protein type: Protein kinase, CAMK; Kinase, protein; EC 2.7.11.27; Autophagy; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.31; CAMK group; CAMKL family; AMPK subfamily

Cellular Component: AMP-activated protein kinase complex; cytoplasm; nucleus

Molecular Function: [acetyl-CoA carboxylase] kinase activity; [hydroxymethylglutaryl-CoA reductase (NADPH)] kinase activity; AMP-activated protein kinase activity; ATP binding; histone serine kinase activity; metal ion binding

Biological Process: autophagy; cellular response to glucose starvation; cholesterol biosynthetic process; fatty acid biosynthetic process; glucose homeostasis; negative regulation of TOR signaling pathway; protein amino acid phosphorylation; regulation of macroautophagy; regulation of transcription, DNA-dependent; rhythmic process; transcription, DNA-dependent; Wnt receptor signaling pathway