Human Apolipoprotein A2 (APOA2) RTU ELISA Kit | APOA2 rtu elisa kit

Human Apolipoprotein A2 (APOA2) ELISA Kit

Cat. Num. AAA457691

• Gene Names: APOA2; apoAII; Apo-AII; ApoA-II
• Reactivity: Human
• Synonyms: Apolipoprotein A2 (APOA2); Human Apolipoprotein A2 (APOA2) ELISA Kit; Apo-A2; Apolipoprotein A-II; ProapoA-II; Truncated apolipoprotein A-II; APOA2 rtu elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of APOA2. No significant cross-reactivity or interference between APOA2 and analogues was observed.
• Sequence Length: 100

• Samples: Serum, plasma or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5-4000ng/mL
• Sensitivity: < 25.4ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level APOA2 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level APOA2 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOA2 rtu elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of APOA2 in human serum, plasma or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to APOA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to APOA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain APOA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of APOA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502149
• NCBI GeneID: 336
• NCBI Accession #: NP_001634.1
• NCBI GenBank Nucleotide #: NM_001643.1
• UniProt Accession #: P02652; B2R524
• Molecular Weight: 11,175 Da
• NCBI Official Full Name: apolipoprotein A-II preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A2
• NCBI Official Symbol: APOA2
• NCBI Official Synonym Symbols: apoAII; Apo-AII; ApoA-II
• NCBI Protein Information: apolipoprotein A-II
• UniProt Protein Name: Apolipoprotein A-II
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA2
• UniProt Synonym Gene Names: Apo-AII; ApoA-II; ProapoA-II

NCBI Description

This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. [provided by RefSeq, Jul 2008]

Uniprot Description

May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.