Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with IgG Monoclonal Antibody (IG266))
Mouse anti-Human IgG (Immunoglobulin Gamma Heavy Chain) Monoclonal Antibody | anti-IGHG antibody
IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Mouse Monoclonal Antibody
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Synonyms
IgG (Immunoglobulin Gamma Heavy Chain); Monoclonal Antibody; IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Mouse Monoclonal Antibody; G1m Marker; G2m Marker; G3m Marker; G4m Marker; HDC; Heavy Chain Disease Protein; Human Immunglobulin G; Ig gamma1/2/3/4 Chain C Region; IGHG1; IGHG2; IGHG3; IGHG4; Immunoglobulin Heavy Constant ? 1/2/3/4; anti-IGHG antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a, kappa
Clone Number
IG266
Specificity
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Sequence Length
330
Applicable Applications for anti-IGHG antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Cellular Localization
Cytoplasm, Cell Surface and Secreted
Immunogen
Purified human Ig Gamma Chain
Hu-Chromosome Location
14q32.33
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with IgG Monoclonal Antibody (IG266))
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Tonsil stained with IgG Monoclonal Antibody (IG266))
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Product Notes
The IGHG (Catalog #AAA438489) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The IgG (Immunoglobulin Gamma Heavy Chain) (B-Cell Marker) Mouse Monoclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IgG (Immunoglobulin Gamma Heavy Chain) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin. Flow Cytometry (0.5-1ug/million cells in 0.1ml) Immunofluorescence (0.5-1ug/ml) Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Researchers should empirically determine the suitability of the IGHG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG (Immunoglobulin Gamma Heavy Chain), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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