Rat Apolipoprotein A2 (APOA2) ELISA Kit | APOA2 elisa kit

Rat Apolipoprotein A2 (APOA2) ELISA Kit

Cat. Num. AAA2702133

• Gene Names: APOA2; apoAII; Apo-AII; ApoA-II
• Reactivity: Rat
• Synonyms: Apolipoprotein A2 (APOA2); Rat Apolipoprotein A2 (APOA2) ELISA Kit; Apo-A2; Apolipoprotein A-II; ProapoA-II; Truncated apolipoprotein A-II; APOA2 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Apolipoprotein A2 (APOA2).; No significant cross-reactivity or interference between Apolipoprotein A2 (APOA2) and analogues was observed.
• Sequence Length: 100

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma and Other Biological Fluids
• Detection Range: 6.25-400ng/mL
• Sensitivity: < 2.57ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Apolipoprotein A2 (APOA2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Apolipoprotein A2 (APOA2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOA2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A2 (APOA2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A2 (APOA2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A2 (APOA2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Apolipoprotein A2 (APOA2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502149
• NCBI GeneID: 336
• NCBI Accession #: NP_001634.1
• NCBI GenBank Nucleotide #: NM_001643.1
• UniProt Accession #: P02652; B2R524
• Molecular Weight: 11,175 Da
• NCBI Official Full Name: apolipoprotein A-II preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A2
• NCBI Official Symbol: APOA2
• NCBI Official Synonym Symbols: apoAII; Apo-AII; ApoA-II
• NCBI Protein Information: apolipoprotein A-II
• UniProt Protein Name: Apolipoprotein A-II
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA2
• UniProt Synonym Gene Names: Apo-AII; ApoA-II; ProapoA-II

NCBI Description

This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. [provided by RefSeq, Jul 2008]

Uniprot Description

May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.