Rat Apolipoprotein A-II ELISA Kit | Apoa2 elisa kit

Rat Apolipoprotein A-II ELISA Kit

Cat. Num. AAA762128

• Gene Names: APOA2; apoAII; Apo-AII; ApoA-II
• Reactivity: Rat
• Synonyms: Apolipoprotein A-II; Rat Apolipoprotein A-II ELISA Kit; Apoa2/Apo-AII/ApoA-II/apoAII/Apo-A2/Apolipoprotein A-II/Alp-2/Hdl-1/Apolipoprotein A2; Apoa2 elisa kit

• Reactivity: Rat
• Sequence Length: 100

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 6.25-400ng/ml
• Sensitivity: 3.75ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Apoa2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Rat Apoa2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Apoa2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Apoa2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Apoa2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4502149
• NCBI GeneID: 336
• NCBI Accession #: NP_001634.1
• NCBI GenBank Nucleotide #: NM_001643.1
• Molecular Weight: 11,175 Da
• NCBI Official Full Name: apolipoprotein A-II preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-II
• NCBI Official Symbol: APOA2
• NCBI Official Synonym Symbols: apoAII; Apo-AII; ApoA-II
• NCBI Protein Information: apolipoprotein A-II
• UniProt Protein Name: Apolipoprotein A-II
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA2
• UniProt Synonym Gene Names: Apo-AII; ApoA-II; ProapoA-II
• UniProt Entry Name: APOA2_HUMAN

NCBI Description

This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA2: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. Belongs to the apolipoprotein A2 family.

Protein type: Endoplasmic reticulum; Lipid-binding; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: chylomicron; cytosol; early endosome; endoplasmic reticulum lumen; extracellular region

Molecular Function: apolipoprotein receptor binding; cholesterol binding; cholesterol transporter activity; high-density lipoprotein binding; lipase inhibitor activity; lipid binding; lipid transporter activity; phosphatidylcholine binding; phospholipid binding; protein binding; protein heterodimerization activity; protein homodimerization activity

Biological Process: cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; diacylglycerol catabolic process; lipoprotein biosynthetic process; lipoprotein metabolic process; negative regulation of cholesterol transport; negative regulation of cytokine secretion during immune response; negative regulation of lipase activity; negative regulation of lipid catabolic process; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; phospholipid catabolic process; phospholipid efflux; positive regulation of interleukin-8 biosynthetic process; positive regulation of lipid catabolic process; protein amino acid oxidation; regulation of protein stability; response to glucose stimulus; retinoid metabolic process; reverse cholesterol transport; triacylglycerol metabolic process

Disease: Hypercholesterolemia, Familial