Rabbit p190-A RhoGAP Polyclonal Antibody | anti-ARHGAP35 antibody

p190-A RhoGAP

Cat. Num. AAA617467

• Gene Names: ARHGAP35; GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP
• Reactivity: Hamster, Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: p190-A RhoGAP; Polyclonal Antibody; Anti -p190-A RhoGAP; anti-ARHGAP35 antibody

• Host: Rabbit
• Reactivity: Hamster, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total p190-A RhoGAP protein. Species Crossreactivity: Human, mouse, rat, hamster.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, 50% glycerol.

• Applicable Applications for anti-ARHGAP35 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic peptide corresponding to corresponding to central residues of human p190-A RhoGAP protein conjugated to KLH (Q9NRY4).
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from various cell types using MBS617467 p190-A RhoGAP Antibody.)

Related Product Information for anti-ARHGAP35 antibody

The Rho family of small GTPases, including Rho, Rac and Cdc42, are key regulators of diverse biological processes such as cytoskeletal organization, cell growth and differentiation, transcriptional regulation, and cell adhesion/motility (1,2). The activities of these proteins are controlled by GTP binding such that in the GTP-bound state they are active, and in the GDP-bound state they become inactive. Three classes of regulatory proteins control the activity of the GTPases by balancing the levels of GTP/GDP binding: the guanine nucleotide exchange factors (GEFs), which promote the active state by facilitating the exchange of GDP for GTP; the guanine nucleotide dissociation inhibitors (GDIs), which sequester the GDP-bound forms and may regulate their intracellular localization; and the GTPase activating proteins (GAPs), which increase GTP hydrolysis to promote the inactive state. The p190 RhoGAP family consists of two related proteins, p190-A and p190-B, which are both widely expressed and contain an amino-terminal GTPase domain and a carboxy-terminal RhoGAP catalytic domain. Mice lacking p190-B RhoGAP show excessive Rho activation and a reduction in activation of the transcription factor CREB (3). Phenotypes of these mice are similar to those of CREB knockouts, with reduced cell size, as well as defects in thymus and neural development (3). Cells deficient in p190-B also display defective adipogenesis, suggesting this pathway is critical for the "adipogenesismyogenesis switch." (4).

There is increasing evidence that p190 undergoes tyrosine phosphorylation, which activates its GAP domain (4-6). Levels of tyrosine phosphorylation are enhanced by Src overexpression (5,6). IGF-1 treatment downregulates Rho through phosphorylation and activation of p190-B RhoGAP, thereby enhancing IGF signaling implicated in adipogenesis (4). p190-A has been characterized as a tumor suppressor based on its ability to reverse v-Ha-Ras-induced malignancy in cultured cells (7). Loss or rearrangement of the chromosomal region containing the gene for p190-A has been implicated in the development of some human tumors (8). p190-A can also bind the mitogen-inducible transcription factor TFII-I sequestering it in the cytoplasm and inhibiting its activity. Phosphorylation of p190-A at Tyr308 reduces its affinity for TFII-I, relieving the inhibition (9). p190-A can also inhibit growth factor-induced gliomas in mice (10) and affect cleavage furrow formation and cytokinesis in cultured cells (11).

Product Categories/Family for anti-ARHGAP35 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 530416350
• NCBI GeneID: 2909
• NCBI Accession #: XP_005258880.1
• NCBI GenBank Nucleotide #: XM_005258823.1
• UniProt Accession #: Q9NRY4; Q14452; Q9C0E1; A7E2A4
• Molecular Weight: 170,514 Da
• NCBI Official Full Name: rho GTPase-activating protein 35 isoform X1
• NCBI Official Synonym Full Names: Rho GTPase activating protein 35
• NCBI Official Symbol: ARHGAP35
• NCBI Official Synonym Symbols: GRF-1; GRLF1; P190A; P190-A; p190RhoGAP; p190ARhoGAP
• NCBI Protein Information: rho GTPase-activating protein 35; rho GAP p190A; glucocorticoid receptor repression factor 1; glucocorticoid receptor DNA binding factor 1; glucocorticoid receptor DNA-binding factor 1
• UniProt Protein Name: Rho GTPase-activating protein 35
• Protein Family: Rho GTPase-activating protein
• UniProt Gene Name: ARHGAP35
• UniProt Synonym Gene Names: GRF1; GRLF1; KIAA1722; GRF-1; p190-A
• UniProt Entry Name: RHG35_HUMAN

NCBI Description

The human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. The amino acid sequence deduced from the cDNA sequences show the presence of three sequence motifs characteristic of a zinc finger and one motif suggestive of a leucine zipper in which 1 cysteine is found instead of all leucines. The GRLF1 enhances the homologous down-regulation of wild-type hGR gene expression. Biochemical analysis suggests that GRLF1 interaction is sequence specific and that transcriptional efficacy of GRLF1 is regulated through its interaction with specific sequence motif. The level of expression is regulated by glucocorticoids. [provided by RefSeq, Jul 2008]

Uniprot Description

GRF-1: the human glucocorticoid receptor DNA binding factor, which associates with the promoter region of the glucocorticoid receptor gene (hGR gene), is a repressor of glucocorticoid receptor transcription. May participate in the regulation of retinal development and degeneration. May transduce signals from p21-ras to the nucleus, acting via the ras GTP-ase activating protein (GAP). May also act as a tumor suppressor. Two splice variant isoforms have been described.

Protein type: Tumor suppressor; GAPs, Rac/Rho; Motility/polarity/chemotaxis; GAPs; Transcription factor

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleus; cytosol; actin cytoskeleton

Molecular Function: DNA binding; GTP binding; transcription corepressor activity

Biological Process: axon guidance; regulation of cell shape; regulation of small GTPase mediated signal transduction; camera-type eye development; negative regulation of Rho protein signal transduction; transcription, DNA-dependent; small GTPase mediated signal transduction; forebrain development; neural tube closure; negative regulation of vascular permeability; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent