NAMPT active protein

Nampt, Recombinant, Human (Visfatin/PBEF, PBEF1, Pre-B Cell Colony Enhancing Factor 1, Nicotinamide Phosphoribosyltransferase)

Cat. Num. AAA635704

• Gene Names: NAMPT; VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
• Purity: Highly Purified; >95% (SDS-PAGE)
• Synonyms: Nampt; Recombinant; Human (Visfatin/PBEF; PBEF1; Pre-B Cell Colony Enhancing Factor 1; Nicotinamide Phosphoribosyltransferase); NAMPT active protein

• Host: Human, HEK 293 cells
• Purity/Purification: Highly Purified; >95% (SDS-PAGE)
• Form/Format: Supplied as a lyophilized powder from a 0.2um-filtered solution in PBS, pH 7.2.
• Sequence: Extracellular human Nampt (visfatin/PBEF) (aa 1-491) is fused at the N-terminus to a DYKDDDDK-tag.

• Biological Activity: Shows adipogenic effects in stimulated differentiating 3T3-L1 cells.
• Preparation and Storage: Short-term Storage: +4 degree C; Long-term Storage: -20 degree C; Working aliquots are stable for up to 3 months when stored at -20 degree C.

Related Product Information for NAMPT active protein

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kD adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

Product Categories/Family for NAMPT active protein

Molecular Biology; MB-Obesity Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 5031977
• NCBI GeneID: 10135
• NCBI Accession #: NP_005737.1
• NCBI GenBank Nucleotide #: NM_005746.2
• UniProt Accession #: P43490; Q3KQV0; Q8WW95; A4D0Q9; A4D0R0
• Molecular Weight: ~52kD (SDS-PAGE)
• NCBI Official Full Name: nicotinamide phosphoribosyltransferase
• NCBI Official Synonym Full Names: nicotinamide phosphoribosyltransferase
• NCBI Official Symbol: NAMPT
• NCBI Official Synonym Symbols: VF; PBEF; PBEF1; VISFATIN; 1110035O14Rik
• NCBI Protein Information: nicotinamide phosphoribosyltransferase; NAmPRTase; pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; pre-B-cell colony-enhancing factor 1
• UniProt Protein Name: Nicotinamide phosphoribosyltransferase
• Protein Family: Nicotinamide phosphoribosyltransferase
• UniProt Gene Name: NAMPT
• UniProt Synonym Gene Names: PBEF; PBEF1; NAmPRTase; Nampt
• UniProt Entry Name: NAMPT_HUMAN

NCBI Description

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]

Uniprot Description

PBEF: an ubiquitous protein originally described as a cytokine which acts on early B-lineage precursor cells. Other reports indicate that PBEF is not a cytokine-like secreted protein but an intracellular protein associated with the cell cycle. Contains a catalytically active nicotinate phosphoribosyltransferase domain, an enzyme involved in nicotinamide adenine dinucleotide (NAD) biosynthesis.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cell cycle regulation; Cytokine; Transferase; EC 2.4.2.12

Chromosomal Location of Human Ortholog: 7q22.3

Cellular Component: extracellular space; nucleus; cytosol

Molecular Function: protein binding; protein homodimerization activity; nicotinate-nucleotide diphosphorylase (carboxylating) activity; cytokine activity; drug binding; nicotinamide phosphoribosyltransferase activity

Biological Process: circadian rhythm; vitamin metabolic process; positive regulation of smooth muscle cell proliferation; female pregnancy; signal transduction; response to organic cyclic substance; NAD biosynthetic process; cell-cell signaling; NAD metabolic process; nicotinamide metabolic process; positive regulation of cell proliferation; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; water-soluble vitamin metabolic process; positive regulation of nitric-oxide synthase biosynthetic process