Rabbit anti-Mouse CD2 Polyclonal Antibody | anti-CD2 antibody

Anti-CD2 Antibody, Rabbit Polyclonal

Cat. Num. AAA8111716

• Gene Names: Cd2; Ly37; LFA-2; Ly-37
• Reactivity: Mouse
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: CD2; Polyclonal Antibody; Anti-CD2 Antibody; Rabbit Polyclonal; CD2 Antibody; Rabbit PAb; Antigen Affinity Purified; CD2 molecule; Anti-LFA-2 Antibody; Anti-Ly-37 Antibody; Anti-Ly37 Antibody; anti-CD2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Mouse CD2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CD2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant Mouse CD2 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse CD2 (rM CD2). CD2 specific IgG was purified by Mouse CD2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-CD2 rabbit polyclonal antibody at 1:500 dilution Lane A: Jurkat Whole Cell Lysate Lane B: Mouse brain tissue lysate Lane C: Mouse spleen tissue lysate Lane D: Mouse brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:39 kDa Observed band size:44 kDa)

Related Product Information for anti-CD2 antibody

T-cell surface antigen CD2, also known as T-cell surface antigen T11/Leu-5, and SRBC, is a single-pass type I membrane protein. It contains one Ig-like C2-type domain and one Ig-like V-type domain. CD2 is a cell adhesion molecule expressed on T cells and is recognized as a target for CD48 (rats) and CD58 (humans). CD2 has been shown to set quantitative thresholds in T cell activation both in vivo and in vitro. Further, intracellular CD2 signaling pathways and networks are being discovered by the identification of several cytosolic tail binding proteins. CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain of CD2 is implicated in the signaling function. The complex of CD2 and CD58 also plays an important role in enhancing the adhesion of T lymphocytes to target cells, and promoting hyperplasia and activation of T lymphocytes. As a cell surface glycoprotein, CD2 expressed on most human T cells and natural killer (NK) cells and plays an important role in mediating cell adhesion in both T-lymphocytes and in signal transduction.

References

Yang JJ, et al. (2001) Structural biology of the cell adhesion protein CD2: alternatively folded states and structure-function relation. Curr Protein Pept Sci. 2(1): 1-17.
Wilkins AL, et al. (2003) Structural biology of the cell adhesion protein CD2: from molecular recognition to protein folding and design. Curr Protein Pept Sci. 4(5): 367-73.
McNerney ME, et al. (2006) The CD2 family of natural killer cell receptors. Curr Top Microbiol Immunol. 298: 91-120.

NCBI and Uniprot Product Information

• NCBI GI #: 7304949
• NCBI GeneID: 12481
• NCBI Accession #: NP_038514.1
• NCBI GenBank Nucleotide #: NM_013486.2
• UniProt Accession #: P08920; Q61394
• Molecular Weight: 38,415 Da
• NCBI Official Full Name: T-cell surface antigen CD2
• NCBI Official Synonym Full Names: CD2 antigen
• NCBI Official Symbol: Cd2
• NCBI Official Synonym Symbols: Ly37; LFA-2; Ly-37
• NCBI Protein Information: T-cell surface antigen CD2
• UniProt Protein Name: T-cell surface antigen CD2
• Protein Family: CD2
• UniProt Gene Name: Cd2
• UniProt Synonym Gene Names: Ly-37; Ly-37
• UniProt Entry Name: CD2_MOUSE

Uniprot Description

CD2: CD2 interacts with lymphocyte function-associated antigen (LFA-3) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T- cells, the cytoplasmic domain is implicated in the signaling function.

Protein type: Membrane protein, integral; Apoptosis; Cell surface

Cellular Component: internal side of plasma membrane; cell surface; protein complex; anchored to plasma membrane; membrane; integral to membrane; extracellular region; intercellular junction; lipid raft; external side of plasma membrane

Molecular Function: protein binding; protein homodimerization activity; protein self-association; receptor activity; glycoprotein binding; receptor tyrosine kinase binding; protein kinase binding; antigen binding; receptor binding

Biological Process: heterotypic cell-cell adhesion; cell-cell adhesion; T cell activation; cell adhesion; positive regulation of tumor necrosis factor production