Human Transcription Factor Binding To IGHM Enhancer 3 (TFE3) ELISA Kit | TFE3 elisa kit

Human Transcription Factor Binding To IGHM Enhancer 3 (TFE3) ELISA Kit

Cat. Num. AAA2706753

• Gene Names: TFE3; TFEA; RCCP2; RCCX1; bHLHe33
• Reactivity: Human
• Synonyms: Transcription Factor Binding To IGHM Enhancer 3 (TFE3); Human Transcription Factor Binding To IGHM Enhancer 3 (TFE3) ELISA Kit; RCCP2; TFEA; BHLHE33; Transcription Tactor E Family Member A; Class E basic helix-loop-helix protein 33; Transcription factor E3; TFE3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Transcription Factor Binding To IGHM Enhancer 3 (TFE3).; No significant cross-reactivity or interference between Transcription Factor Binding To IGHM Enhancer 3 (TFE3) and analogues was observed.
• Sequence Length: 109

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.118ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transcription Factor Binding To IGHM Enhancer 3 (TFE3) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transcription Factor Binding To IGHM Enhancer 3 (TFE3) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TFE3 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription Factor Binding To IGHM Enhancer 3 (TFE3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcription Factor Binding To IGHM Enhancer 3 (TFE3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcription Factor Binding To IGHM Enhancer 3 (TFE3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transcription Factor Binding To IGHM Enhancer 3 (TFE3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19684176
• NCBI GeneID: 7030
• UniProt Accession #: P19532; Q5JU74; Q92757; Q92758; Q99964; A8MZL6
• Molecular Weight: 11,363 Da
• NCBI Official Full Name: Transcription factor binding to IGHM enhancer 3
• NCBI Official Synonym Full Names: transcription factor binding to IGHM enhancer 3
• NCBI Official Symbol: TFE3
• NCBI Official Synonym Symbols: TFEA; RCCP2; RCCX1; bHLHe33
• NCBI Protein Information: transcription factor E3
• UniProt Protein Name: Transcription factor E3
• Protein Family: Transcription factor
• UniProt Gene Name: TFE3
• UniProt Synonym Gene Names: BHLHE33; bHLHe33

NCBI Description

This gene encodes a basic helix-loop-helix domain-containing transcription factor that binds MUE3-type E-box sequences in the promoter of genes. The encoded protein promotes the expression of genes downstream of transforming growth factor beta (TGF-beta) signaling. This gene may be involved in chromosomal translocations in renal cell carcinomas and other cancers, resulting in the production of fusion proteins. Translocation partners include PRCC (papillary renal cell carcinoma), NONO (non-POU domain containing, octamer-binding), and ASPSCR1 (alveolar soft part sarcoma chromosome region, candidate 1), among other genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

Transcription factor that specifically recognizes and binds E-box sequences (5'-CANNTG-3'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. In association with TFEB, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and thymus-dependent humoral immunity. Specifically recognizes the MUE3 box, a subset of E-boxes, present in the immunoglobulin enhancer. It also binds very well to a USF/MLTF site.