Typical Testing Data/Standard Curve (for reference only)
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Human Xaa-Pro Dipeptidase/Prolidase (PEPD) ELISA Kit | PEPD elisa kit
Human Xaa-Pro Dipeptidase/Prolidase (PEPD) ELISA Kit
Reactivity
Human
Synonyms
Xaa-Pro Dipeptidase/Prolidase (PEPD); Human Xaa-Pro Dipeptidase/Prolidase (PEPD) ELISA Kit; PEPD elisa kit
Reactivity
Human
Assay Type
Sandwich
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
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Typical Testing Data/Standard Curve (for reference only)
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Related Product Information for PEPD elisa kit
Principle of the Assay: Human Xaa-Pro Dipeptidase/Prolidase (PEPD) ELISA Kit employs a two-site sandwich ELISA to quantitate PEPD in samples. An antibody specific for PEPD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PEPD present is bound by the immobilized antibody. After removing any unbound substances, HRP-Conjugated Human PEPD detection antibody is added to the wells. Following a wash to remove any unbound HRP reagent, a Chromogen solution is added to the wells and color develops in proportion to the amount of PEPD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background/Introduction: Xaa-Pro dipeptidase is a cytosolic dipeptidase that hydrolyzes dipeptides with proline or hydroxyproline at the carboxy terminus (but not Pro-Pro). It is important in collagen metabolism because of the high levels of aminoacids.The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.
Background/Introduction: Xaa-Pro dipeptidase is a cytosolic dipeptidase that hydrolyzes dipeptides with proline or hydroxyproline at the carboxy terminus (but not Pro-Pro). It is important in collagen metabolism because of the high levels of aminoacids.The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.
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Product Notes
The Human PEPD (Catalog #AAA9714914) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714914 ELISA Kit recognizes Human PEPD. It is sometimes possible for the material contained within the vial of "Xaa-Pro Dipeptidase/Prolidase (PEPD), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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