PEPD recombinant protein

PEPD, 1-493aa, Human, His tag, E Coli

Cat. Num. AAA206056

• Gene Names: PEPD; PROLIDASE
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: PEPD; 1-493aa; Human; His tag; E Coli; Xaa-Pro dipeptidase isoform 1; PROLIDASE; PEPD recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid, In Phosphate buffered saline (pH 7.4) containing 10% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAAATGP SFWLGNETLK VPLALFALNR QRLCERLRKN PAVQAGSIVV LQGGEETQRY CTDTGVLFRQ ESFFHWAFGV TEPGCYGVID VDTGKSTLFV PRLPASHATW MGKIHSKEHF KEKYAVDDVQ YVDEIASVLT SQKPSVLLTL RGVNTDSGSV CREASFDGIS KFEVNNTILH PEIVECRVFK TDMELEVLRY TNKISSEAHR EVMKAVKVGM KEYELESLFE HYCYSRGGMR HSSYTCICGS GENSAVLHYG HAGAPNDRTI QNGDMCLFDM GGEYYCFASD ITCSFPANGK FTADQKAVYE AVLRSSRAVM GAMKPGVWWP DMHRLADRIH LEELAHMGIL SGSVDAMVQA HLGAVFMPHG LGHFLGIDVH DVGGYPEGVE RIDEPGLRSL RTARHLQPGM VLTVEPGIYF IDHLLDEALA DPARASFLNR EVLQRFRGFG GVRIEEDVVV TDSGIELLTC VPRTVEEIEA CMAGCDKAFT PFSGPK
• Sequence Length: 493

• Applicable Applications for PEPD recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PEPD recombinant protein

PEPD also known as Xaa-Pro dipeptidase isoform1. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Recombinant human PEPD, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PEPD recombinant protein

Metabolism

References

Toprak G., et al. (2013) Eur Rev Med Pharmacol Sci 17 (17), 2302-2309; Surazynski A., et al. (2013) Mol. Cell. Biochem. 379 (1-2), 29-36

NCBI and Uniprot Product Information

• NCBI GI #: 149589008
• NCBI GeneID: 5184
• NCBI Accession #: NP_000276
• NCBI GenBank Nucleotide #: NM_000285.3
• UniProt Accession #: P12955; Q8TBN9; Q9BT75; A8K3Z1; A8K416; A8K696; A8MX47; B4DDB7; B4DGJ1; E9PCE8
• Molecular Weight: 56.9kDa (516aa)
• NCBI Official Full Name: xaa-Pro dipeptidase isoform 1
• NCBI Official Synonym Full Names: peptidase D
• NCBI Official Symbol: PEPD
• NCBI Official Synonym Symbols: PROLIDASE
• NCBI Protein Information: xaa-Pro dipeptidase
• UniProt Protein Name: Xaa-Pro dipeptidase
• Protein Family: Dipeptidase
• UniProt Gene Name: PEPD
• UniProt Synonym Gene Names: PRD; X-Pro dipeptidase; Prolidase

NCBI Description

This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

Uniprot Description

Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen.