Human Wingless Type MMTV Integration Site Family, Member 11 (WNT11) ELISA Kit | WNT11 elisa kit

Human Wingless Type MMTV Integration Site Family, Member 11 (WNT11) ELISA Kit

Cat. Num. AAA451431

• Gene Names: WNT11; HWNT11
• Reactivity: Human
• Synonyms: Wingless Type MMTV Integration Site Family; Member 11 (WNT11); Human Wingless Type MMTV Integration Site Family; Member 11 (WNT11) ELISA Kit; WNT11 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of WNT11. No significant cross-reactivity or interference between WNT11 and analogues was observed.
• Sequence Length: 354

• Samples: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.058ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT11 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT11 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for WNT11 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of WNT11 in human tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT11. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to WNT11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of WNT11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 17017974
• NCBI GeneID: 7481
• NCBI Accession #: NP_004617.2
• NCBI GenBank Nucleotide #: NM_004626.2
• UniProt Accession #: O96014; Q14DE8; Q8WZ98; B2R8Z6
• Molecular Weight: 39,179 Da
• NCBI Official Full Name: protein Wnt-11
• NCBI Official Synonym Full Names: Wnt family member 11
• NCBI Official Symbol: WNT11
• NCBI Official Synonym Symbols: HWNT11
• NCBI Protein Information: protein Wnt-11
• UniProt Protein Name: Protein Wnt-11
• Protein Family: Protein
• UniProt Gene Name: WNT11

NCBI Description

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 97%, 85%, and 63% amino acid identity with mouse, chicken, and Xenopus Wnt11 protein, respectively. This gene may play roles in the development of skeleton, kidney and lung, and is considered to be a plausible candidate gene for High Bone Mass Syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.