Typical Testing Data/Standard Curve (for reference only)
Mouse Wingless Type MMTV Integration Site Family, Member 11 (WNT11) ELISA Kit | WNT11 elisa kit
Mouse Wingless Type MMTV Integration Site Family, Member 11 (WNT11) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to WNT11. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to WNT11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of WNT11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 97%, 85%, and 63% amino acid identity with mouse, chicken, and Xenopus Wnt11 protein, respectively. This gene may play roles in the development of skeleton, kidney and lung, and is considered to be a plausible candidate gene for High Bone Mass Syndrome. [provided by RefSeq, Jul 2008]
Uniprot Description
WNT11: Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Belongs to the Wnt family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 11q13.5
Cellular Component: extracellular space; proteinaceous extracellular matrix; cytoplasm; extracellular region
Molecular Function: frizzled binding; protein kinase activator activity
Biological Process: response to nutrient levels; cell fate commitment; adrenal gland development; positive regulation of apoptosis; embryonic skeletal development; positive regulation of transcription, DNA-dependent; cloacal septation; palate development; Wnt receptor signaling pathway through beta-catenin; positive regulation of transforming growth factor-beta2 production; protein amino acid phosphorylation; bone mineralization; osteoblast differentiation; neuron differentiation; positive regulation of protein kinase activity; positive regulation of stress fiber formation; artery morphogenesis; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; negative regulation of cell migration; negative regulation of apoptosis; positive regulation of cell migration
Research Articles on WNT11
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Product Notes
The Mouse WNT11 wnt11 (Catalog #AAA2025032) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2025032 ELISA Kit recognizes Mouse WNT11. It is sometimes possible for the material contained within the vial of "Wingless Type MMTV Integration Site Family, Member 11 (WNT11), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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