Human Virus Inhibitory Protein (Viperin) ELISA Kit | RSAD2 elisa kit

Human Virus Inhibitory Protein (Viperin) ELISA Kit

Cat. Num. AAA2023571

• Gene Names: RSAD2; cig5; vig1; cig33; 2510004L01Rik
• Reactivity: Human
• Synonyms: Virus Inhibitory Protein (Viperin); Human Virus Inhibitory Protein (Viperin) ELISA Kit; Cig5; RSAD2; vig1; Radical S-Adenosyl Methionine Domain Containing 2; Viperin; Endoplasmic Reticulum-Associated; Interferon-Inducible; Cytomegalovirus-induced gene 5; RSAD2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Viperin. No significant cross-reactivity or interference between Viperin and analogues was observed.
• Sequence Length: 361

• Samples: Tissue homogenates, cell lysates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.055ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Viperin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Viperin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RSAD2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of Viperin in human tissue homogenates, cell lysates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to Viperin. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Viperin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Viperin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Viperin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RSAD2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 17974550
• NCBI GeneID: 91543
• UniProt Accession #: Q8WXG1; Q8WVI4
• Molecular Weight: 42,170 Da
• NCBI Official Full Name: viperin
• NCBI Official Synonym Full Names: radical S-adenosyl methionine domain containing 2
• NCBI Official Symbol: RSAD2
• NCBI Official Synonym Symbols: cig5; vig1; cig33; 2510004L01Rik
• NCBI Protein Information: radical S-adenosyl methionine domain-containing protein 2
• UniProt Protein Name: Radical S-adenosyl methionine domain-containing protein 2
• Protein Family: Radical S-adenosyl methionine domain-containing protein
• UniProt Gene Name: RSAD2
• UniProt Entry Name: RSAD2_HUMAN

Uniprot Description

RSAD2: Interferon-inducible iron-sulfur (4FE-4S) cluster- binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), and human immunodeficiency virus (HIV-1). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphospate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63'-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins. Belongs to the radical SAM superfamily. RSAD2 family.

Chromosomal Location of Human Ortholog: 2p25.2

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; mitochondrial inner membrane; lipid particle

Molecular Function: protein binding; protein self-association; 4 iron, 4 sulfur cluster binding; metal ion binding; catalytic activity

Biological Process: positive regulation of toll-like receptor 7 signaling pathway; negative regulation of protein secretion; ossification; viral reproduction; negative regulation of viral genome replication; CD4-positive, alpha beta T cell differentiation; metabolic process; cytokine and chemokine mediated signaling pathway; response to virus; positive regulation of toll-like receptor 9 signaling pathway; regulation of ossification; defense response to virus