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Western Blot (WB) (Western blot analysis of MeCP2-S421* in mouse brain, cerebellum tissue and HepG2 cell line lysates (35ug/lane). CP2 (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse MeCP2 Polyclonal Antibody | anti-MeCP2 antibody

MeCP2 (S421) (MECP2, Methyl-CpG-binding protein 2) (PE)

Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MeCP2; Polyclonal Antibody; MeCP2 (S421) (MECP2; Methyl-CpG-binding protein 2) (PE); MECP2; Methyl-CpG-binding protein 2; anti-MeCP2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-MeCP2 antibody
FLISA, Western Blot (WB)
Application Notes
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
MeCP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding S421 of human MECP2.
Conjugate
PE
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MeCP2-S421* in mouse brain, cerebellum tissue and HepG2 cell line lysates (35ug/lane). CP2 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of MeCP2-S421* in mouse brain, cerebellum tissue and HepG2 cell line lysates (35ug/lane). CP2 (arrow) was detected using the purified Pab.)
Related Product Information for anti-MeCP2 antibody
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of some cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.
Product Categories/Family for anti-MeCP2 antibody
References
dos Santos, J.M., et al., Neurosci. Lett. 379(1):13-16 (2005). Ylisaukko-Oja, T., et al., Am J Med Genet A 132(2):121-124 (2005). Schanen, C., et al., Am J Med Genet A 126(2):129-140 (2004). Shibayama, A., et al., Am. J. Med. Genet. B Neuropsychiatr. Genet. 128(1):50-53 (2004). Fang, J.Y., et al., World J. Gastroenterol. 10(23):3394-3398 (2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
52,427 Da
NCBI Official Full Name
methyl-CpG-binding protein 2
NCBI Official Symbol
MECP2
NCBI Protein Information
methyl-CpG-binding protein 2
UniProt Protein Name
Methyl-CpG-binding protein 2
UniProt Gene Name
MECP2
UniProt Synonym Gene Names
MeCp-2 protein; MeCp2

Uniprot Description

Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).

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Product Notes

The MeCP2 mecp2 (Catalog #AAA6319891) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MeCP2 (S421) (MECP2, Methyl-CpG-binding protein 2) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MeCP2 can be used in a range of immunoassay formats including, but not limited to, FLISA, Western Blot (WB). FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MeCP2 mecp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MeCP2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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