Mouse Viperin ELISA Kit | Rsad2 elisa kit

Mouse Viperin ELISA Kit

Cat. Num. AAA456929

• Gene Names: Rsad2; Vig1; cig5; 2510004L01Rik
• Reactivity: Mouse
• Synonyms: Viperin; Mouse Viperin ELISA Kit; Cig5; RSAD2; vig1; Radical S-Adenosyl Methionine Domain Containing 2; Endoplasmic Reticulum-Associated; Interferon-Inducible; Cytomegalovirus-induced gene 5; Rsad2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Viperin. No significant cross-reactivity or interference between Viperin and analogues was observed.
• Sequence Length: 362

• Samples: Serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.072ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Viperin were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Viperin were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Rsad2 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of Viperin in mouse serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Viperin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Viperin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Viperin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Viperin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 31543946
• NCBI GeneID: 58185
• NCBI Accession #: NP_067359.2
• NCBI GenBank Nucleotide #: NM_021384.4
• UniProt Accession #: Q8CBB9; Q3U5I6; Q3U5T4; Q3U622; Q3U627; Q3U7I6; Q3U7M1; Q3U8F4; Q3U8U7; Q3U941; Q3U977; Q3U9E1
• Molecular Weight: 41,524 Da
• NCBI Official Full Name: radical S-adenosyl methionine domain-containing protein 2
• NCBI Official Synonym Full Names: radical S-adenosyl methionine domain containing 2
• NCBI Official Symbol: Rsad2
• NCBI Official Synonym Symbols: Vig1; cig5; 2510004L01Rik
• NCBI Protein Information: radical S-adenosyl methionine domain-containing protein 2
• UniProt Protein Name: Radical S-adenosyl methionine domain-containing protein 2
• Protein Family: Radical S-adenosyl methionine domain-containing protein
• UniProt Gene Name: Rsad2

Uniprot Description

RSAD2: Interferon-inducible iron-sulfur (4FE-4S) cluster- binding antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Can inhibit a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), and human immunodeficiency virus (HIV-1). Displays antiviral activity against influenza A virus by inhibiting the budding of the virus from the plasma membrane by disturbing the lipid rafts. This is accomplished, at least in part, through binding and inhibition of the enzyme farnesyl diphospate synthase (FPPS), which is essential for the biosynthesis of isoprenoid-derived lipids. Promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating Lys-63'-linked ubiquitination of IRAK1. Plays a role in CD4+ T-cells activation and differentiation. Facilitates T-cell receptor (TCR)-mediated GATA3 activation and optimal T helper 2 (Th2) cytokine production by modulating NFKB1 and JUNB activities. Can inhibit secretion of soluble proteins. Belongs to the radical SAM superfamily. RSAD2 family.

Chromosomal Location of Human Ortholog: 12|12 A2

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; fibrillar center; lipid droplet; membrane; mitochondrial inner membrane; mitochondrial outer membrane; mitochondrion

Molecular Function: 4 iron, 4 sulfur cluster binding; catalytic activity; iron-sulfur cluster binding; metal ion binding; protein binding; protein self-association

Biological Process: CD4-positive, alpha beta T cell differentiation; defense response to virus; immune system process; innate immune response; negative regulation of protein secretion; negative regulation of viral genome replication; ossification; positive regulation of toll-like receptor 7 signaling pathway; positive regulation of toll-like receptor 9 signaling pathway; regulation of ossification; response to virus