Human Thymidine Kinase 1, Soluble (TK1) ELISA Kit | TK1 elisa kit

Human Thymidine Kinase 1, Soluble (TK1) ELISA Kit

Cat. Num. AAA2021703

• Gene Names: TK1; TK2
• Reactivity: Human
• Synonyms: Thymidine Kinase 1; Soluble (TK1); Human Thymidine Kinase 1; Soluble (TK1) ELISA Kit; TK2; Thymidine kinase; cytosolic; TK1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Thymidine Kinase 1, Soluble (TK1).; No significant cross-reactivity or interference between Thymidine Kinase 1, Soluble (TK1) and analogues was observed.
• Sequence Length: 234

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 78-5,000pg/mL
• Sensitivity: < 29pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Thymidine Kinase 1, Soluble (TK1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Thymidine Kinase 1, Soluble (TK1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TK1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymidine Kinase 1, Soluble (TK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymidine Kinase 1, Soluble (TK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymidine Kinase 1, Soluble (TK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymidine Kinase 1, Soluble (TK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for TK1 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 52632392
• NCBI GeneID: 7083
• UniProt Accession #: P04183; Q969V0; Q9UMG9; B2RC58
• Molecular Weight: 25,469 Da
• NCBI Official Full Name: Thymidine kinase 1, soluble
• NCBI Official Synonym Full Names: thymidine kinase 1, soluble
• NCBI Official Symbol: TK1
• NCBI Official Synonym Symbols: TK2
• NCBI Protein Information: thymidine kinase, cytosolic
• UniProt Protein Name: Thymidine kinase, cytosolic
• Protein Family: Thymidine kinase
• UniProt Gene Name: TK1
• UniProt Entry Name: KITH_HUMAN

Uniprot Description

TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Protein type: EC 2.7.1.21; Xenobiotic Metabolism - drug metabolism - other enzymes; Kinase, other; Nucleotide Metabolism - pyrimidine

Chromosomal Location of Human Ortholog: 17q23.2-q25.3

Cellular Component: cytosol

Molecular Function: identical protein binding; nucleoside kinase activity; zinc ion binding; thymidine kinase activity; ATP binding

Biological Process: response to nutrient levels; skeletal muscle cell proliferation; nucleobase, nucleoside and nucleotide metabolic process; response to toxin; liver development; deoxyribonucleoside monophosphate biosynthetic process; protein homotetramerization; fetal process involved in parturition; response to cortisol stimulus; pyrimidine base metabolic process; gut development; response to copper ion; nucleotide biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; pyrimidine nucleoside salvage; thymidine metabolic process; phosphorylation