Porcine Thymidine kinase, cytosolic (TK1) ELISA Kit | TK1 elisa kit

Porcine Thymidine kinase, cytosolic (TK1) ELISA Kit

Cat. Num. AAA7251686

• Gene Names: TK1; TK2
• Reactivity: Porcine
• Synonyms: Thymidine kinase; cytosolic (TK1); Porcine Thymidine kinase; cytosolic (TK1) ELISA Kit; TK1 elisa kit

• Reactivity: Porcine

• Samples: Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TK1 elisa kit

Intended Uses: This TK1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine TK1. This ELISA kit for research use only!

Principle of the Assay: TK1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-TK1 antibody and an TK1-HRP conjugate. The assay sample and buffer are incubated together with TK1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TK1 concentration since TK1 from samples and TK1-HRP conjugate compete for the anti-TK1 antibody binding site. Since the number of sites is limited, as more sites are occupied by TK1 from the sample, fewer sites are left to bind TK1HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TK1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 164698438
• NCBI GeneID: 7083
• NCBI Accession #: NP_003249.3
• NCBI GenBank Nucleotide #: NM_003258.4
• Molecular Weight: 25,469 Da
• NCBI Official Full Name: thymidine kinase, cytosolic
• NCBI Official Synonym Full Names: thymidine kinase 1, soluble
• NCBI Official Symbol: TK1
• NCBI Official Synonym Symbols: TK2
• NCBI Protein Information: thymidine kinase, cytosolic; thymidine kinase-1; thymidine kinase 1 soluble isoform
• UniProt Protein Name: Thymidine kinase, cytosolic
• Protein Family: Thymidine kinase
• UniProt Gene Name: TK1
• UniProt Entry Name: KITH_HUMAN

Uniprot Description

TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Nucleotide Metabolism - pyrimidine; Kinase, other; EC 2.7.1.21

Chromosomal Location of Human Ortholog: 17q23.2-q25.3

Cellular Component: cytosol

Molecular Function: identical protein binding; nucleoside kinase activity; zinc ion binding; thymidine kinase activity; ATP binding

Biological Process: response to nutrient levels; skeletal muscle cell proliferation; nucleobase, nucleoside and nucleotide metabolic process; response to toxin; liver development; protein homotetramerization; fetal process involved in parturition; deoxyribonucleoside monophosphate biosynthetic process; response to cortisol stimulus; response to copper ion; pyrimidine base metabolic process; gut development; nucleotide biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; pyrimidine nucleoside salvage; thymidine metabolic process; phosphorylation