Rat Thymidine kinase, cytosolic ELISA Kit | Tk1 elisa kit

Rat Thymidine kinase, cytosolic ELISA Kit

Cat. Num. AAA761924

• Gene Names: TK1; TK2
• Reactivity: Rat
• Synonyms: Thymidine kinase; cytosolic; Rat Thymidine kinase; cytosolic ELISA Kit; Tk1/thymidine kinase 1 soluble isoform/thymidine kinase/cytosolic/thymidine kinase-1/TK2; Tk1 elisa kit

• Reactivity: Rat
• Sequence Length: 234

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Tk1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat Tk1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Tk1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Tk1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Tk1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 164698438
• NCBI GeneID: 7083
• NCBI Accession #: NP_003249.3
• NCBI GenBank Nucleotide #: NM_003258.4
• Molecular Weight: 25,469 Da
• NCBI Official Full Name: thymidine kinase, cytosolic
• NCBI Official Synonym Full Names: thymidine kinase 1, soluble
• NCBI Official Symbol: TK1
• NCBI Official Synonym Symbols: TK2
• NCBI Protein Information: thymidine kinase, cytosolic
• UniProt Protein Name: Thymidine kinase, cytosolic
• Protein Family: Thymidine kinase
• UniProt Gene Name: TK1
• UniProt Entry Name: KITH_HUMAN

Uniprot Description

TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.

Protein type: Nucleotide Metabolism - pyrimidine; Kinase, other; EC 2.7.1.21; Xenobiotic Metabolism - drug metabolism - other enzymes

Chromosomal Location of Human Ortholog: 17q23.2-q25.3

Cellular Component: cytosol

Molecular Function: ATP binding; identical protein binding; nucleoside kinase activity; protein binding; thymidine kinase activity; zinc ion binding

Biological Process: deoxyribonucleoside monophosphate biosynthetic process; fetal process involved in parturition; gut development; liver development; nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; nucleotide biosynthetic process; phosphorylation; protein homotetramerization; pyrimidine base metabolic process; pyrimidine nucleoside salvage; response to copper ion; response to cortisol stimulus; response to nutrient levels; response to toxin; skeletal muscle cell proliferation; thymidine metabolic process