Western Blot (WB)
(Western blot analysis of extracts from various samples, using SAMM50 Antibody. Lane 1: Rat kidney, treated with blocking peptide; Lane 2: Rat kidney; Lane 3: HepG2; Lane 4: Mouse lung.)
Rabbit SAMM50 Polyclonal Antibody | anti-SAMM50 antibody
SAMM50 Antibody
Gene Names
SAMM50; OMP85; SAM50; TOB55; TRG-3; CGI-51; YNL026W
Reactivity
Human, Mouse, RatPredicted: Pig, Zebrafish, Bovine, Horse, Sheep, Chicken
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
SAMM50; Polyclonal Antibody; SAMM50 Antibody; CGI 51; CGI-51; FLJ35825; FLJ42905; FLJ99036; OMP85; SAM50; SAM50_HUMAN; Samm50; sorting and assembly machinery 50kDa; sorting and assembly machinery component 50 homolog (S. cerevisiae); Sorting and assembly machinery component 50 homolog; sorting and assembly machinery component 50; S. cerevisiae; homolog of; TOB55; Transformation-related gene 3 protein; TRG-3; YNL026W; anti-SAMM50 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Chicken
Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Chicken
Clonality
Polyclonal
Isotype
IgG
Specificity
SAMM50 Antibody detects endogenous levels of SAMM50.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
469
Applicable Applications for anti-SAMM50 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human SAMM50.
Fragment
Fab Fragment
Conjugate
Unconjugated
Subcellular Location
Mitochondrion
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from various samples, using SAMM50 Antibody. Lane 1: Rat kidney, treated with blocking peptide; Lane 2: Rat kidney; Lane 3: HepG2; Lane 4: Mouse lung.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using SAMM50 Antibody. Lane 1: Rat kidney, treated with blocking peptide; Lane 2: Rat kidney; Lane 3: HepG2; Lane 4: Mouse lung.)
Western Blot (WB)
(Western blot analysis of extracts from A431 cells, using SAMM50 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from A431 cells, using SAMM50 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-SAMM50 antibody
Description: Plays a crucial role in the maintenance of the structure of mitochondrial cristae and the proper assembly of the mitochondrial respiratory chain complexes (PubMed:22252321, PubMed:25781180). Required for the assembly of TOMM40 into the TOM complex (PubMed:15644312).
Subunit Structure: Associates with the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MINOS1/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13 (PubMed:25781180, PubMed:25997101). This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9 (PubMed:25781180, PubMed:25997101). The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex (PubMed:25997101). Interacts with CHCHD3/MIC19 (PubMed:21081504).
Similarity: Its C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure. Belongs to the SAM50/omp85 family.
Subunit Structure: Associates with the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MINOS1/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and QIL1/MIC13 (PubMed:25781180, PubMed:25997101). This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9 (PubMed:25781180, PubMed:25997101). The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex (PubMed:25997101). Interacts with CHCHD3/MIC19 (PubMed:21081504).
Similarity: Its C-terminal part seems to contain many membrane-spanning sided beta-sheets, that have the potential to adopt a transmembrane beta-barrel type structure. Belongs to the SAM50/omp85 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed: 52 kDa
Predicted: 52 kDa
Predicted: 52 kDa
NCBI Official Full Name
Sorting and assembly machinery component 50 homolog
NCBI Official Synonym Full Names
SAMM50 sorting and assembly machinery component
NCBI Official Symbol
SAMM50
NCBI Official Synonym Symbols
OMP85; SAM50; TOB55; TRG-3; CGI-51; YNL026W
NCBI Protein Information
sorting and assembly machinery component 50 homolog
UniProt Protein Name
Sorting and assembly machinery component 50 homolog
Protein Family
UniProt Gene Name
SAMM50
UniProt Synonym Gene Names
SAM50; TRG-3
UniProt Entry Name
SAM50_HUMAN
NCBI Description
This gene encodes a component of the Sorting and Assembly Machinery (SAM) of the mitochondrial outer membrane. The Sam complex functions in the assembly of beta-barrel proteins into the outer mitochondrial membrane.[provided by RefSeq, Jun 2011]
Uniprot Description
SAMM50: May be required for the assembly pathway of mitochondrial outer membrane proteins. Belongs to the SAM50/omp85 family.
Protein type: Membrane protein, multi-pass; Mitochondrial
Chromosomal Location of Human Ortholog: 22q13.31
Cellular Component: mitochondrial outer membrane; mitochondrion; mitochondrial inner membrane; integral to membrane
Molecular Function: protein binding
Biological Process: cellular protein metabolic process; protein targeting to mitochondrion; protein import into mitochondrial outer membrane
Research Articles on SAMM50
Similar Products
Product Notes
The SAMM50 samm50 (Catalog #AAA9611480) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SAMM50 Antibody reacts with Human, Mouse, Rat Predicted: Pig, Zebrafish, Bovine, Horse, Sheep, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's SAMM50 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the SAMM50 samm50 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SAMM50, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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