Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human TGF-beta-inducible early response gene-1 (TIEG1) Standard Curve.)
Human TGF-beta-Inducible Early Response Gene-1 (TIEG1) ELISA Kit | TIEG1 elisa kit
Human TGF-beta-Inducible Early Response Gene-1 (TIEG1) ELISA Kit
Gene Names
KLF10; EGRA; TIEG; TIEG1; EGR-alpha
Reactivity
Human
Synonyms
TGF-beta-Inducible Early Response Gene-1 (TIEG1); Human TGF-beta-Inducible Early Response Gene-1 (TIEG1) ELISA Kit; KLF10; EGRA; TIEG; TIEG1; TGFB inducible early growth response; TIEG1 elisa kit
Reactivity
Human
Specificity
Human TGF-beta-inducible early response gene-1 (TIEG1) ELISA Kit has high sensitivity and excellent specificity for detection of Human KLF10. No significant cross-reactivity or interference between Human KLF10 and analogues was observed.
Sequence Length
469
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human TGF-beta-inducible early response gene-1 (TIEG1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human TGF-beta-inducible early response gene-1 (TIEG1) Standard Curve.)
Related Product Information for TIEG1 elisa kit
Description: This Human TGF-beta-inducible early response gene-1 (TIEG1) ELISA Kit employs a two-site sandwich ELISA to quantitate KLF10 in samples. An antibody specific for KLF10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLF10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLF10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLF10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Tachibana et al. characterized the expression and function in exocrine pancreatic epithelial cells of the TGF-beta-inducible early gene (TIEG), a Kruppel-like zinc finger transcription factor-encoding gene previously isolated from mesodermally derived osteoblastic cells by Subramaniam et al. Tachibana et al. demonstrated that the gene is expressed in both acinar and ductular epithelial cell populations from the exocrine pancreas. In addition, they showed that the expression of TIEG is regulated by TGF-beta-1 (TGFB1) as an early response gene in pancreatic epithelial cell lines. Overexpression of TIEG in a TGF-beta-sensitive epithelial cell line was sufficient to induce apoptosis. Thus, Tachibana et al. concluded that TIEG plays a role in linking TGF-beta-mediated signaling cascades to the regulation of pancreatic epithelial cell growth.
Background: Tachibana et al. characterized the expression and function in exocrine pancreatic epithelial cells of the TGF-beta-inducible early gene (TIEG), a Kruppel-like zinc finger transcription factor-encoding gene previously isolated from mesodermally derived osteoblastic cells by Subramaniam et al. Tachibana et al. demonstrated that the gene is expressed in both acinar and ductular epithelial cell populations from the exocrine pancreas. In addition, they showed that the expression of TIEG is regulated by TGF-beta-1 (TGFB1) as an early response gene in pancreatic epithelial cell lines. Overexpression of TIEG in a TGF-beta-sensitive epithelial cell line was sufficient to induce apoptosis. Thus, Tachibana et al. concluded that TIEG plays a role in linking TGF-beta-mediated signaling cascades to the regulation of pancreatic epithelial cell growth.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
Krueppel-like factor 10 isoform b
NCBI Official Synonym Full Names
Kruppel like factor 10
NCBI Official Symbol
KLF10
NCBI Official Synonym Symbols
EGRA; TIEG; TIEG1; EGR-alpha
NCBI Protein Information
Krueppel-like factor 10
UniProt Protein Name
Krueppel-like factor 10
UniProt Gene Name
KLF10
UniProt Synonym Gene Names
TIEG; TIEG1; TGFB-inducible early growth response protein 1; TIEG-1
UniProt Entry Name
KLF10_HUMAN
NCBI Description
This gene encodes a member of a family of proteins that feature C2H2-type zinc finger domains. The encoded protein is a transcriptional repressor that acts as an effector of transforming growth factor beta signaling. Activity of this protein may inhibit the growth of cancers, particularly pancreatic cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]
Research Articles on TIEG1
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Product Notes
The Human TIEG1 klf10 (Catalog #AAA9715571) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715571 ELISA Kit recognizes Human TIEG1. It is sometimes possible for the material contained within the vial of "TGF-beta-Inducible Early Response Gene-1 (TIEG1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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