Human Kruppel-like factor 10 ELISA Kit | KLF10 elisa kit

Human TGF-beta-inducible early response gene-1, TIEG1 ELISA Kit

Cat. Num. AAA703882

• Gene Names: KLF10; EGRA; TIEG; TIEG1; EGR-alpha
• Reactivity: Human
• Synonyms: Kruppel-like factor 10; Human TGF-beta-inducible early response gene-1; TIEG1 ELISA Kit; EGRA; TIEG; TIEG1; TGFB inducible early growth response; KLF10 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human TIEG1. No significant cross-reactivity or interference between human TIEG1 and analogues was observed.
• Sequence Length: 469

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 34.38 pg/ml -2200 pg/ml.
• Sensitivity: The minimum detectable dose of human TIEG1 is typically less than 8.6 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for KLF10 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TIEG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIEG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIEG1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIEG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 73760403
• NCBI GeneID: 7071
• NCBI Accession #: NP_001027453.1
• NCBI GenBank Nucleotide #: NM_001032282.3
• UniProt Accession #: Q13118; O75411; Q503B2; A8MVH0; B2R794; L0R4P6; L0R679
• Molecular Weight: 52,555 Da
• NCBI Official Full Name: Krueppel-like factor 10 isoform b
• NCBI Official Synonym Full Names: Kruppel-like factor 10
• NCBI Official Symbol: KLF10
• NCBI Official Synonym Symbols: EGRA; TIEG; TIEG1; EGR-alpha
• NCBI Protein Information: Krueppel-like factor 10; early growth response-alpha; zinc finger transcription factor TIEG; TGFB-inducible early growth response protein 1; transforming growth factor-beta-inducible early growth response protein 1
• UniProt Protein Name: Krueppel-like factor 10
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF10
• UniProt Synonym Gene Names: TIEG; TIEG1; TGFB-inducible early growth response protein 1; TIEG-1
• UniProt Entry Name: KLF10_HUMAN

NCBI Description

This gene encodes a member of a family of proteins that feature C2H2-type zinc finger domains. The encoded protein is a transcriptional repressor that acts as an effector of transforming growth factor beta signaling. Activity of this protein may inhibit the growth of cancers, particularly pancreatic cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]

Uniprot Description

Function: Transcriptional repressor involved in the regulation of cell growth. Inhibits cell growth. Binds to the consensus sequence 5'-GGTGTG-3'.1 PublicationManual assertion based on experiment in:Ref.1