Human A2M ELISA Kit | A2M elisa kit

Human A2M ELISA Kit

Cat. Num. AAA825043

• Gene Names: A2M; A2MD; CPAMD5; FWP007; S863-7
• Reactivity: Human
• Applications: ELISA
• Synonyms: A2M; Human A2M ELISA Kit; CPAMD5; Alpha-2-macroglobulin; Alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5; A2M elisa kit

• Reactivity: Human
• Specificity: The Human A2M ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human A2M proteins within the range of 625 pg/ml - 40000 pg/ml.
• Sequence Length: 1474

• Applicable Applications for A2M elisa kit: Sandwich ELISA (SE)
• Samples: Cell Culture Supernatants, Serum, Plasma
• Assay Type: Quantitative Sandwich
• Sensitivity: <20 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for A2M elisa kit

Principle of the Assay: The MyBioSource' Human A2M ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human A2M in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human A2M coated on a 96-well plate. Standards and samples are pipetted into the wells and A2M present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human A2M antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of A2M bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.!!Background/Introduction: Alpha-2-macroglobulin, also known as A2M or CPAMD5 is a large plasma protein found in the blood. This gene is mapped to 12p13.31. Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. This gene is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase.

NCBI and Uniprot Product Information

• NCBI GI #: 66932947
• NCBI GeneID: 2
• NCBI Accession #: NP_000005.2
• NCBI GenBank Nucleotide #: NM_000014.4
• UniProt Accession #: P01023; Q13677; Q59F47; Q5QTS0; Q68DN2; Q6PIY3; Q6PN97
• Molecular Weight: 163,291 Da
• NCBI Official Full Name: alpha-2-macroglobulin
• NCBI Official Synonym Full Names: alpha-2-macroglobulin
• NCBI Official Symbol: A2M
• NCBI Official Synonym Symbols: A2MD; CPAMD5; FWP007; S863-7
• NCBI Protein Information: alpha-2-macroglobulin; alpha-2-M; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
• UniProt Protein Name: Alpha-2-macroglobulin
• Protein Family: Alpha-2-macroglobulin
• UniProt Gene Name: A2M
• UniProt Synonym Gene Names: CPAMD5; Alpha-2-M
• UniProt Entry Name: A2MG_HUMAN

NCBI Description

Alpha-2-macroglobulin is a protease inhibitor and cytokine transporter. It inhibits many proteases, including trypsin, thrombin and collagenase. A2M is implicated in Alzheimer disease (AD) due to its ability to mediate the clearance and degradation of A-beta, the major component of beta-amyloid deposits. [provided by RefSeq, Jul 2008]

Uniprot Description

A2M: Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family.

Protein type: Secreted; Inhibitor; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: extracellular region; cytosol

Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; enzyme binding; protease binding; growth factor binding; interleukin-8 binding; interleukin-1 binding; calcium-dependent protein binding; tumor necrosis factor binding; receptor binding

Biological Process: negative regulation of complement activation, lectin pathway; platelet activation; extracellular matrix disassembly; extracellular matrix organization and biogenesis; regulation of small GTPase mediated signal transduction; platelet degranulation; small GTPase mediated signal transduction; stem cell differentiation; blood coagulation; blood coagulation, intrinsic pathway

Disease: Alzheimer Disease; Alpha-2-macroglobulin Deficiency