Human STAT1 ELISA Kit | STAT1 elisa kit

Human STAT1 (Signal Transducer and Activator of Transcription 1) ELISA Kit

Cat. Num. AAA2507040

• Gene Names: Stat1; AA408197; 2010005J02Rik
• Reactivity: Human
• Synonyms: STAT1; Human STAT1 (Signal Transducer and Activator of Transcription 1) ELISA Kit; STAT1 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human SRP9 in samples. No Significant cross-reactivity or interference between Human SRP9 and analogues was observed.
• Sequence Length: 749

• Samples: Serum, Plasma And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.13-5000 pg/mL
• Sensitivity: 46.88 pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human SRP9 were tested 20 times on one plate, respectively
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human SRP9 were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STAT1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human SRP9 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SRP9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SRP9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SRP9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human SRP9. You can calculate the concentration of Human SRP9 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 458710
• NCBI GeneID: 20846
• UniProt Accession #: P42225
• Molecular Weight: 87,197 Da
• NCBI Official Full Name: Stat1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 1
• NCBI Official Symbol: Stat1
• NCBI Official Synonym Symbols: AA408197; 2010005J02Rik
• NCBI Protein Information: signal transducer and activator of transcription 1
• UniProt Protein Name: Signal transducer and activator of transcription 1
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: Stat1
• UniProt Entry Name: STAT1_MOUSE

Uniprot Description

STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Cellular Component: nucleoplasm; axon; nuclear chromatin; dendrite; cytoplasm; nucleolus; nucleus

Molecular Function: identical protein binding; protein binding; signal transducer activity; protein homodimerization activity; enzyme binding; DNA binding; protein phosphatase 2A binding; sequence-specific DNA binding; double-stranded DNA binding; tumor necrosis factor receptor binding; transcription factor activity; CCR5 chemokine receptor binding

Biological Process: transcription from RNA polymerase II promoter; response to peptide hormone stimulus; response to cAMP; blood circulation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; response to lipopolysaccharide; signal transduction; response to exogenous dsRNA; tumor necrosis factor-mediated signaling pathway; regulation of transcription, DNA-dependent; positive regulation of cell proliferation; negative regulation of viral protein levels in host cell; lipopolysaccharide-mediated signaling pathway; response to nutrient; caspase activation; response to drug; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; JAK-STAT cascade; negative regulation of angiogenesis; response to bacterium; cellular response to insulin stimulus; response to mechanical stimulus; response to cytokine stimulus; negative regulation of endothelial cell proliferation; positive regulation of transcription from RNA polymerase II promoter