Rabbit STAT1 Polyclonal Antibody | anti-STAT1 antibody

Anti-STAT1 Antibody

Cat. Num. AAA419367

• Gene Names: Stat1; AA408197; 2010005J02Rik
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purification
• Synonyms: STAT1; Polyclonal Antibody; Anti-STAT1 Antibody; anti-STAT1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Western blot analysis of various cell lines, using STAT1 antibody.
• Purity/Purification: Affinity Purification
• Form/Format: Liquid
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 749

• Applicable Applications for anti-STAT1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50- 1:200
• Immunogen: Recombinant protein of human STAT1
• Storage Buffer: PBS with 0.02% sodium azide,50% glycerol, pH7.3.
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Western Blot (WB)

Related Product Information for anti-STAT1 antibody

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-+/- and IFN-3 (2, 3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1+/- (91 kDa) and the splice variant Stat12 (84 kDa). In most cells, both isoforms are activated by IFN-+/-, but only Stat1+/- is activated by IFN-3. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-+/- and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

NCBI and Uniprot Product Information

• NCBI GI #: 458710
• NCBI GeneID: 20846
• Molecular Weight: 87,197 Da
• NCBI Official Full Name: Stat1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 1
• NCBI Official Symbol: Stat1
• NCBI Official Synonym Symbols: AA408197; 2010005J02Rik
• NCBI Protein Information: signal transducer and activator of transcription 1
• UniProt Protein Name: Signal transducer and activator of transcription 1
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: Stat1

Uniprot Description

STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1 C1.1|1 26.81 cM

Cellular Component: axon; cell-cell adherens junction; cytoplasm; dendrite; nuclear chromatin; nucleolus; nucleoplasm; nucleus; perinuclear region of cytoplasm

Molecular Function: CCR5 chemokine receptor binding; DNA binding; double-stranded DNA binding; enzyme binding; nuclear hormone receptor binding; protein homodimerization activity; protein phosphatase 2A binding; sequence-specific DNA binding; transcription factor activity; tumor necrosis factor receptor binding

Biological Process: blood circulation; caspase activation; cellular response to insulin stimulus; cytokine and chemokine mediated signaling pathway; JAK-STAT cascade; lipopolysaccharide-mediated signaling pathway; negative regulation of angiogenesis; negative regulation of endothelial cell proliferation; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of viral protein levels in host cell; positive regulation of cell proliferation; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; response to bacterium; response to cAMP; response to cytokine stimulus; response to drug; response to exogenous dsRNA; response to lipopolysaccharide; response to mechanical stimulus; response to nutrient; response to peptide hormone stimulus; transcription, DNA-dependent; tumor necrosis factor-mediated signaling pathway