Human RNASE3/ECP ELISA Kit | RNASE3/ECP elisa kit

Human RNASE3/ECP (Ribonuclease A3/Eosinophil Cationic Protein) ELISA Kit

Cat. Num. AAA2500372

• Gene Names: RNASE3; ECP; RNS3
• Reactivity: Human
• Synonyms: RNASE3/ECP; Human RNASE3/ECP (Ribonuclease A3/Eosinophil Cationic Protein) ELISA Kit; RNASE3/ECP elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human RNASE in samples. No Significant cross-reactivity or interference between Human RNASE and analogues was observed.
• Sequence Length: 160

• Samples: Serum, Plasma And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.13-5000 pg/mL
• Sensitivity: 46.88 pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human RNASE were tested 20 times on one plate, respectively
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human RNASE were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RNASE3/ECP elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human RNASE concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RNASE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human RNASE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human RNASE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human RNASE. You can calculate the concentration of Human RNASE in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 64654615
• NCBI GeneID: 6037
• UniProt Accession #: P12724; Q4VBC1; Q8WTP7; Q8WZ62; Q9GZN9
• Molecular Weight: 18,385 Da
• NCBI Official Full Name: Ribonuclease, RNase A family, 3 (eosinophil cationic protein)
• NCBI Official Synonym Full Names: ribonuclease, RNase A family, 3
• NCBI Official Symbol: RNASE3
• NCBI Official Synonym Symbols: ECP; RNS3
• NCBI Protein Information: eosinophil cationic protein; RNase 3; cytotoxic ribonuclease; ribonuclease 3
• UniProt Protein Name: Eosinophil cationic protein
• UniProt Gene Name: RNASE3
• UniProt Synonym Gene Names: ECP; RNS3; ECP; RNase 3
• UniProt Entry Name: ECP_HUMAN

NCBI Description

The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. The protein exhibits antimicrobial activity against pathogenic bacteria [provided by RefSeq, Oct 2014]

Uniprot Description

RNASE3: Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. Possesses a wide variety of biological activities. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. Belongs to the pancreatic ribonuclease family.

Protein type: Ribonuclease; Secreted; Secreted, signal peptide; EC 3.1.27.-

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: extracellular space; extracellular region

Molecular Function: nucleic acid binding; endonuclease activity; ribonuclease activity

Biological Process: defense response to Gram-positive bacterium; innate immune response in mucosa; RNA catabolic process; antibacterial humoral response