Rabbit anti-Human, Mouse LRRK2 Polyclonal Antibody | anti-LRRK2 antibody

LRRK2 (Leucine-rich Repeat Kinase 2, Leucine-rich Repeat Serine/threonine Protein Kinase 2, AURA17, Dardarin, PARK8, RIPK7, ROCO2) (PE)

Cat. Num. AAA6485461

• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: LRRK2; Polyclonal Antibody; LRRK2 (Leucine-rich Repeat Kinase 2; Leucine-rich Repeat Serine/threonine Protein Kinase 2; AURA17; Dardarin; PARK8; RIPK7; ROCO2) (PE); anti-LRRK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LRRK2. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-LRRK2 antibody: FLISA, Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 930-961 of human LRRK2, UniProt Accession #Q5S007.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse lung tissue lysates (35ug/lane) usingMBS623581.This demonstrates that MBS623581 detected the Park8 (LRRK2) protein (arrow).)

Western Blot (WB)

(Western Blot analysis detected over-expressed human LRRK2 protein usingMBS623581.)

Immunofluorescence (IF)

(Immunofluorescence analysis is Tau-stable SY5Y cell image stained for endogenous LRRK (green) by MBS623581 (affinity purified), phosphorylated tau (red, monoclonal AT8) and nuclear staining by DAPI.)

Related Product Information for anti-LRRK2 antibody

Parkinson is the second most common neurodegenerative disease after Alzheimers. About 1 percent of people over the age of 65 and 3 percent of people over the age of 75 are affected by the disease. The mutation is the most common cause of Parkinson's disease identified to date. LRRK2, a genetic mutation, was recently found linked to about 5 percent of inherited cases of Parkinson's disease. By high-resolution recombination mapping and candidate gene sequencing in 46 families, 6 disease-segregating mutations (5 missense and 1 putative splice site mutation). It may be central to the pathogenesis of several major neurodegenerative disorders associated with parkinsonism. LRRK2 belongs to the ROCO protein family and includes a protein kinase domain of the MAPKKK class and several other major functional domains.

Product Categories/Family for anti-LRRK2 antibody

Antibodies; Protein Kinases

References

1. Shen J. Neuron. 2004. 44:575. 2. Paisan-Ruiz C, et al. Neuron. 2004. 44(4):595. 3. Zimprich A, et al. Neuron. 2004. 44(4):601. 4. Wszolek ZK, et al. Neurology. 2004. 62(9):1619. 5. Park YJ, et al. Transplant Proc. 2004. 36(2):353. 6.Zimprich A, et al. Am J Hum Genet. 2004. 74:11. 7.Bonifati V. Lancet Neurol. 2002. 1:83. 8.Funayama M, et al. Ann Neurol. 2002. 51:296.

NCBI and Uniprot Product Information

• NCBI GI #: 89035471
• Molecular Weight: 280.863
• Protein Family: Leucine-rich repeat serine/threonine-protein kinase