Rabbit RGS10 Polyclonal Antibody | anti-RGS10 antibody

Rabbit RGS10 Antibody

Cat. Num. AAA2640

• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunofluorescence, Immunocytochemistry
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: RGS10; Polyclonal Antibody; Rabbit RGS10 Antibody; Regulator of G-protein signaling 10;RGS10; anti-RGS10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: RGS10 Antibody detects endogenous levels of total RGS10
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 181

• Applicable Applications for anti-RGS10 antibody: ELISA (EIA), Western Blot (WB), Immunofluroescence (IF), Immunocytochemistry (ICC)
• Application Notes: WB 1:500~1:1000; IHC: 1:50~1:200; IF/ICC: 1:100-1:500
• Immunogen: A synthesized peptide
• Subcellular Location: Cytosol; Nucleus; Nucleus
• Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Preparation and Storage: Store at -20 °C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from rat brain, using RGS10Ab.)

Immunohistochemistry (IHC)

(At 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the Ab for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit Ab was used as the secondary.)

NCBI and Uniprot Product Information

• NCBI GI #: 52694755
• NCBI GeneID: 6001
• NCBI Accession #: NP_001005339.1
• NCBI GenBank Nucleotide #: NM_001005339.1
• UniProt Accession #: O43665; Q6IAZ6; Q96GN0; A8K408; B1AMR8
• Molecular Weight: 20 KD
• NCBI Official Full Name: regulator of G-protein signaling 10 isoform a
• NCBI Official Synonym Full Names: regulator of G-protein signaling 10
• NCBI Official Symbol: RGS10
• NCBI Protein Information: regulator of G-protein signaling 10; regulator of G-protein signalling 10
• UniProt Protein Name: Regulator of G-protein signaling 10
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS10
• UniProt Synonym Gene Names: RGS10
• UniProt Entry Name: RGS10_HUMAN

NCBI Description

Regulator of G protein signaling (RGS) family members are regulatory molecules that act as GTPase activating proteins (GAPs) for G alpha subunits of heterotrimeric G proteins. RGS proteins are able to deactivate G protein subunits of the Gi alpha, Go alpha and Gq alpha subtypes. They drive G proteins into their inactive GDP-bound forms. Regulator of G protein signaling 10 belongs to this family. All RGS proteins share a conserved 120-amino acid sequence termed the RGS domain. This protein associates specifically with the activated forms of the two related G-protein subunits, G-alphai3 and G-alphaz but fails to interact with the structurally and functionally distinct G-alpha subunits. Regulator of G protein signaling 10 protein is localized in the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RGS10: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Associates specifically with the activated forms of the G protein subunits G(i)-alpha and G(z)- alpha but fails to interact with the structurally and functionally distinct G(s)-alpha subunit. Activity on G(z)-alpha is inhibited by palmitoylation of the G-protein.

Protein type: GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 10q25

Cellular Component: cell soma; cytoplasm; dendritic spine; plasma membrane; nerve terminal; nucleus

Molecular Function: GTPase activator activity

Biological Process: positive regulation of GTPase activity