Human Signal Transducer And Activator Of Transcription 5A (STAT5A) ELISA Kit | STAT5A elisa kit

Human Signal Transducer And Activator Of Transcription 5A (STAT5A) ELISA Kit

Cat. Num. AAA2024913

• Gene Names: STAT5A; MGF; STAT5
• Reactivity: Human
• Synonyms: Signal Transducer And Activator Of Transcription 5A (STAT5A); Human Signal Transducer And Activator Of Transcription 5A (STAT5A) ELISA Kit; MGF; STAT5-A; STAT5A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Signal Transducer And Activator Of Transcription 5A (STAT5A).; No significant cross-reactivity or interference between Signal Transducer And Activator Of Transcription 5A (STAT5A) and analogues was observed.
• Sequence Length: 794

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 5A (STAT5A) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 5A (STAT5A) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STAT5A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Transducer And Activator Of Transcription 5A (STAT5A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Transducer And Activator Of Transcription 5A (STAT5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Transducer And Activator Of Transcription 5A (STAT5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Signal Transducer And Activator Of Transcription 5A (STAT5A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for STAT5A elisa kit

Signal transduction; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 20072534
• NCBI GeneID: 6776
• Molecular Weight: 86,949 Da
• NCBI Official Full Name: Signal transducer and activator of transcription 5A
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5A
• NCBI Official Symbol: STAT5A
• NCBI Official Synonym Symbols: MGF; STAT5
• NCBI Protein Information: signal transducer and activator of transcription 5A
• UniProt Protein Name: Signal transducer and activator of transcription 5A
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT5A
• UniProt Synonym Gene Names: STAT5
• UniProt Entry Name: STA5A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Dec 2013]

Uniprot Description

STAT5A: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 fusion protein is essential for the tumorigenesis. Induces the expression of BCL2L1/BCL-X(L) in the mouse, suggesting an antiapoptotic function of this protein. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Oncoprotein; DNA-binding; Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; cytosol

Molecular Function: signal transducer activity; protein binding; transcription factor activity

Biological Process: lactation; succinate metabolic process; oxaloacetate metabolic process; peptidyl-tyrosine phosphorylation; positive regulation of interleukin-2 biosynthetic process; T cell differentiation in the thymus; positive regulation of multicellular organism growth; female pregnancy; positive regulation of mitotic cell cycle; fatty acid metabolic process; positive regulation of activated T cell proliferation; 2-oxoglutarate metabolic process; positive regulation of natural killer cell differentiation; sequestering of lipid; negative regulation of mast cell apoptosis; natural killer cell differentiation; allantoin metabolic process; luteinization; development of secondary male sexual characteristics; regulation of steroid metabolic process; creatinine metabolic process; T cell homeostasis; positive regulation of gamma-delta T cell differentiation; Peyer's patch development; isoleucine metabolic process; negative regulation of erythrocyte differentiation; transcription, DNA-dependent; regulation of multicellular organism growth; valine metabolic process; citrate metabolic process; development of secondary female sexual characteristics; positive regulation of natural killer cell mediated cytotoxicity; JAK-STAT cascade; creatine metabolic process; regulation of transcription from RNA polymerase II promoter; regulation of epithelial cell differentiation; positive regulation of B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; taurine metabolic process; positive regulation of inflammatory response