Human Signal Transducer And Activator Of Transcription 1 (STAT1) ELISA Kit | STAT1 elisa kit

Human Signal Transducer And Activator Of Transcription 1 (STAT1) ELISA Kit

Cat. Num. AAA2024849

• Gene Names: STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
• Reactivity: Human
• Synonyms: Signal Transducer And Activator Of Transcription 1 (STAT1); Human Signal Transducer And Activator Of Transcription 1 (STAT1) ELISA Kit; ISGF-3; STAT91; Transcription Factor ISGF-3 Components p91/p84; STAT1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Signal Transducer And Activator Of Transcription 1 (STAT1).; No significant cross-reactivity or interference between Signal Transducer And Activator Of Transcription 1 (STAT1) and analogues was observed.
• Sequence Length: 750

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 1 (STAT1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 1 (STAT1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STAT1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Transducer And Activator Of Transcription 1 (STAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Transducer And Activator Of Transcription 1 (STAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Transducer And Activator Of Transcription 1 (STAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Signal Transducer And Activator Of Transcription 1 (STAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for STAT1 elisa kit

Signal transduction; Tumor immunity; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 281313277
• NCBI GeneID: 6772
• Molecular Weight: 83,043 Da
• NCBI Official Full Name: signal transducer and activator of transcription 1
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 1, 91kDa
• NCBI Official Symbol: STAT1
• NCBI Official Synonym Symbols: CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
• NCBI Protein Information: signal transducer and activator of transcription 1-alpha/beta
• UniProt Protein Name: Signal transducer and activator of transcription 1-alpha/beta
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT1
• UniProt Entry Name: STAT1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

STAT1: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of certain receptor tyrosine kinases, GPCRs, and receptors for various interleukins and interferons. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2q32.2

Cellular Component: nucleoplasm; axon; nuclear chromatin; dendrite; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: identical protein binding; signal transducer activity; protein binding; enzyme binding; protein homodimerization activity; double-stranded DNA binding; tumor necrosis factor receptor binding; transcription factor activity; CCR5 chemokine receptor binding

Biological Process: transcription from RNA polymerase II promoter; response to peptide hormone stimulus; response to cAMP; viral reproduction; apoptosis; blood circulation; positive regulation of smooth muscle cell proliferation; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; response to exogenous dsRNA; tumor necrosis factor-mediated signaling pathway; positive regulation of mesenchymal cell proliferation; negative regulation of viral protein levels in host cell; lipopolysaccharide-mediated signaling pathway; defense response to virus; response to nutrient; response to drug; cytokine and chemokine mediated signaling pathway; negative regulation of I-kappaB kinase/NF-kappaB cascade; JAK-STAT cascade; regulation of transcription from RNA polymerase II promoter; negative regulation of angiogenesis; cellular response to insulin stimulus; response to hydrogen peroxide; response to mechanical stimulus; response to cytokine stimulus; negative regulation of endothelial cell proliferation; endothelial cell migration; positive regulation of transcription from RNA polymerase II promoter

Disease: Immunodeficiency 31a; Immunodeficiency 31b; Immunodeficiency 31c