Human Signal Transducer And Activator Of Transcription 5A (STAT5A) ELISA Kit | STAT5A elisa kit

Human Signal Transducer And Activator Of Transcription 5A (STAT5A) ELISA Kit

Cat. Num. AAA2020945

• Gene Names: Stat5a; Stat5
• Reactivity: Human
• Synonyms: Signal Transducer And Activator Of Transcription 5A (STAT5A); Human Signal Transducer And Activator Of Transcription 5A (STAT5A) ELISA Kit; STAT5A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Signal Transducer And Activator Of Transcription 5A (STAT5A); No significant cross-reactivity or interference between Signal Transducer And Activator Of Transcription 5A (STAT5A) and analogues was observed.
• Sequence Length: 793

• Samples: Tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 0.313-20ng/mL The standard curve concentrations used for the ELISA's were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL
• Sensitivity: Typically less than 0.112ng/mL.
• Organism Species: Homo sapiens (Human)
• ELISA Type: Sandwich
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 5A (STAT5A) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 5A (STAT5A) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STAT5A elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Transducer And Activator Of Transcription 5A (STAT5A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Transducer And Activator Of Transcription 5A (STAT5A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Transducer And Activator Of Transcription 5A (STAT5A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Signal Transducer And Activator Of Transcription 5A (STAT5A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8394373
• NCBI GeneID: 24918
• NCBI Accession #: NP_058760.1
• NCBI GenBank Nucleotide #: NM_017064.1
• UniProt Accession #: Q62771
• Molecular Weight: 90,833 Da
• NCBI Official Full Name: signal transducer and activator of transcription 5A
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5A
• NCBI Official Symbol: Stat5a
• NCBI Official Synonym Symbols: Stat5
• NCBI Protein Information: signal transducer and activator of transcription 5A; mammary gland factor
• UniProt Protein Name: Signal transducer and activator of transcription 5A
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: Stat5a
• UniProt Synonym Gene Names: Mgf
• UniProt Entry Name: STA5A_RAT

NCBI Description

member of the STAT family of transcription factors; may mediate the biological actions of several interleukin factors (IL2, IL3, IL7) and growth hormones; may be associated with mammary gland function [RGD, Feb 2006]

Uniprot Description

STAT5A: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 fusion protein is essential for the tumorigenesis. Induces the expression of BCL2L1/BCL-X(L) in the mouse, suggesting an antiapoptotic function of this protein. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Oncoprotein; Transcription factor; DNA-binding; Motility/polarity/chemotaxis

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function: protein binding; signal transducer activity; DNA binding; sequence-specific DNA binding; double-stranded DNA binding; protein phosphatase binding; transcription factor activity

Biological Process: mammary gland involution; positive regulation of interleukin-2 biosynthetic process; T cell differentiation in the thymus; positive regulation of multicellular organism growth; female pregnancy; 2-oxoglutarate metabolic process; sequestering of lipid; negative regulation of mast cell apoptosis; allantoin metabolic process; natural killer cell differentiation; regulation of steroid metabolic process; T cell homeostasis; Peyer's patch development; isoleucine metabolic process; transcription, DNA-dependent; valine metabolic process; development of secondary female sexual characteristics; JAK-STAT cascade; regulation of transcription from RNA polymerase II promoter; response to ethanol; regulation of epithelial cell differentiation; positive regulation of transcription from RNA polymerase II promoter; taurine metabolic process; negative regulation of apoptosis; lactation; transcription from RNA polymerase II promoter; succinate metabolic process; response to peptide hormone stimulus; oxaloacetate metabolic process; peptidyl-tyrosine phosphorylation; reproductive process in a multicellular organism; positive regulation of mitotic cell cycle; positive regulation of activated T cell proliferation; signal transduction; fatty acid metabolic process; positive regulation of natural killer cell differentiation; mammary gland development; regulation of transcription, DNA-dependent; luteinization; positive regulation of cell proliferation; development of secondary male sexual characteristics; creatinine metabolic process; positive regulation of gamma-delta T cell differentiation; response to nutrient levels; regulation of cell adhesion; negative regulation of erythrocyte differentiation; cytokine and chemokine mediated signaling pathway; regulation of multicellular organism growth; citrate metabolic process; positive regulation of natural killer cell mediated cytotoxicity; creatine metabolic process; positive regulation of lymphocyte differentiation; positive regulation of B cell differentiation; positive regulation of inflammatory response