Human Signal Transducer And Activator Of Transcription 4 (STAT4) ELISA Kit | STAT4 elisa kit

Human Signal Transducer And Activator Of Transcription 4 (STAT4) ELISA Kit

Cat. Num. AAA2024998

• Gene Names: STAT4; SLEB11
• Reactivity: Human
• Synonyms: Signal Transducer And Activator Of Transcription 4 (STAT4); Human Signal Transducer And Activator Of Transcription 4 (STAT4) ELISA Kit; STAT4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Signal Transducer And Activator Of Transcription 4 (STAT4).; No significant cross-reactivity or interference between Signal Transducer And Activator Of Transcription 4 (STAT4) and analogues was observed.
• Sequence Length: 748

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.108ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 4 (STAT4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Signal Transducer And Activator Of Transcription 4 (STAT4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STAT4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Signal Transducer And Activator Of Transcription 4 (STAT4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Signal Transducer And Activator Of Transcription 4 (STAT4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Signal Transducer And Activator Of Transcription 4 (STAT4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Signal Transducer And Activator Of Transcription 4 (STAT4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for STAT4 elisa kit

Signal transduction; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 345110660
• NCBI GeneID: 6775
• NCBI Accession #: NP_001230764.1
• NCBI GenBank Nucleotide #: NM_001243835.1
• Molecular Weight: 85,941 Da
• NCBI Official Full Name: signal transducer and activator of transcription 4
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 4
• NCBI Official Symbol: STAT4
• NCBI Official Synonym Symbols: SLEB11
• NCBI Protein Information: signal transducer and activator of transcription 4
• UniProt Protein Name: Signal transducer and activator of transcription 4
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT4
• UniProt Entry Name: STAT4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2011]

Uniprot Description

STAT4: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 2q32.2-q32.3

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: signal transducer activity; protein binding; sequence-specific DNA binding; transcription factor activity

Biological Process: cell proliferation; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter; JAK-STAT cascade; protein amino acid phosphorylation

Disease: Systemic Lupus Erythematosus, Susceptibility To, 11