Testing Data
Rabbit Anti-Human DR5 (TRAIL-R2) Secondary Antibody
Rabbit Anti-Human DR5-UNLB
Reactivity
Human
Applications
Flow Cytometry, Western Blot
Purity
Affinity chromatography
Synonyms
Rabbit Anti-Human DR5 (TRAIL-R2); Polyclonal Secondary Antibody; Rabbit Anti-Human DR5-UNLB; Rabbit Anti-Human DR5 - Purified; Unlabeled Antibody; Rabbit Anti-Human DR5 (TRAIL-R2) secondary antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human DR5
DR5, also known as TRAIL-R2, TRICK2 and KILLER, is a widely expressed type I membrane member of the tumor necrosis (TNF) receptor family. Members of this family contain multiple cysteine-rich repeats in their extracellular domain and while the cytoplasmic region is less conserved, they do contain an intracellular 80 amino acid motif known as the 'death domain' (DD).1, 2, 3, 4 DR5 is a receptor for the cytotoxic ligand TRAIL which induces apoptosis and it is the cytoplasmic DD that is responsible for transducing the death process.5, 6 DR5 has also been shown to activate NF- kappa B
DR5, also known as TRAIL-R2, TRICK2 and KILLER, is a widely expressed type I membrane member of the tumor necrosis (TNF) receptor family. Members of this family contain multiple cysteine-rich repeats in their extracellular domain and while the cytoplasmic region is less conserved, they do contain an intracellular 80 amino acid motif known as the 'death domain' (DD).1, 2, 3, 4 DR5 is a receptor for the cytotoxic ligand TRAIL which induces apoptosis and it is the cytoplasmic DD that is responsible for transducing the death process.5, 6 DR5 has also been shown to activate NF- kappa B
Purity/Purification
Affinity chromatography
Form/Format
Purified (UNLB) Antibody
Applicable Applications for Rabbit Anti-Human DR5 (TRAIL-R2) secondary antibody
Flow Cytometry; Western Blot
Application Notes
Research Application(s): Induction of apoptosis
Flow Cytometry Suggested Dilution: <= 3 ug/10^6 cells
Western Blot Suggested Dilution: 1 - 2 ug/mL
Induction of Apoptosis Suggested Dilution: 2.5 ug/mL for 12 hours (10-12% in Jurkat cells)
Flow Cytometry Suggested Dilution: <= 3 ug/10^6 cells
Western Blot Suggested Dilution: 1 - 2 ug/mL
Induction of Apoptosis Suggested Dilution: 2.5 ug/mL for 12 hours (10-12% in Jurkat cells)
Source
Pooled antisera from goats hyperimmunized with S-Tag recombinant protein (see pET-32a-c(+) vector from Novagen)
Immunogen
Recombinant DR5
Characterization
To insure lot-to-lot consistency, each batch of product is tested by flow cytometry and/or western blotting to conform with the characteristics of a standard reference reagent.
Preparation and Storage
The purified antibody (UNLB) is supplied as 0.1 mg purified immunoglobulin in 0.2 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
Reagents are stable for the period shown on the label if stored as directed.
Reagents are stable for the period shown on the label if stored as directed.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
References
1. Schneider, P., J. Bodmer, M. Thome, K. Hofmann, N. Holller, and J. Tschipp. 1997. FEBS Lett. 416:329.
2. MacFarlane, M., M. Ahmad, S.M. Srinivasula, T. Fernandes-Alnemri, G.M. Cohen, and E.S. Alnemri. 1997. J. Biol. Chem. 272:25417.
3. Walczak, H., M.A. Degli-Esposti, R.S. Johnson, P.J. Smolak, J.Y. Waugh, N. Boiani, M.S. Timour, M.J. Gerhart, K.A. Schooley, C.A. Smith, R.G. Goodwin, and C. T. Rauch. 1997. EMBO J. 16:5386.
4. Wu, G.S., T.F. Burns, E.R. McDonald, W. Jiang, R. Meng, I.D. Krantz, G. Kao, D. Gan, J. Zhou, R. Muschel, S.R. Hamilton, N.B. Spinner, S. Markowitz, G. Wu, and W.S. El-Deiry. 1997. Nat. Genet. 17:141.
5. Pitti, R.M., S.A. Marsters, S. Ruppert, C.J. Donahue, A. Moore, and A. Ashkenazi. 1996. J. Biol. Chem. 271:12687.
6. Wiley, S.R., K. Schooley, P.J. Smolak, W.S. Din, C. Huang, J.K. Nicholl, G.R. Sutherland, T.D. Smith, C. Rauch, C.A. Smith, R.G. Goodwin. 1995. Immunity 3:673.
7. Schneider, P., M. Thome, K. Burnes, J. Bodmer, K. Hofmann, T. Kataoka, N. Holler, and J. Tschopp. 1997. Immunity 7:831.
8. Mongkolsapaya, J., J.M. Grimes, N. Chen, X. Xu, D.I. Stuart, E.Y. Jones, and G.R. Screaton. 1999. Nat. Struct. Biol. 6:1048.
9. Sheridan, J.P., S.A. Marsters, R.M. Pitti, A. Gurney, M. Skubatch, D. Baldwin, L. Ramakrishnan, C.L. Gray, K. Baker, W.I. Wood, A.D. Goddard, P. Godowski, and A. Ashkenazi. 1997. Science 277:818.
10. Pan, G., J. Ni, Y. Wei, G. Yu, R. Gentz, and V.M. Dixit. 1997. Science 277:815.
11. Nimmanapalli, R., C.L. Perkins, M. Orlando, E. O'Bryan, D. Nguyen, and K.N. Bhalla. 2001. Cancer Res. 61:759.
12. LaVallee, T.M. X.H. Zhan, M.S. Johnson, C.J. Herstritt, G. Swartz, M.S. Williams, W.A. Hembrough, S.J. Green, and V.S. Pribluda. 2003. Cancer Res. 63:468.
2. MacFarlane, M., M. Ahmad, S.M. Srinivasula, T. Fernandes-Alnemri, G.M. Cohen, and E.S. Alnemri. 1997. J. Biol. Chem. 272:25417.
3. Walczak, H., M.A. Degli-Esposti, R.S. Johnson, P.J. Smolak, J.Y. Waugh, N. Boiani, M.S. Timour, M.J. Gerhart, K.A. Schooley, C.A. Smith, R.G. Goodwin, and C. T. Rauch. 1997. EMBO J. 16:5386.
4. Wu, G.S., T.F. Burns, E.R. McDonald, W. Jiang, R. Meng, I.D. Krantz, G. Kao, D. Gan, J. Zhou, R. Muschel, S.R. Hamilton, N.B. Spinner, S. Markowitz, G. Wu, and W.S. El-Deiry. 1997. Nat. Genet. 17:141.
5. Pitti, R.M., S.A. Marsters, S. Ruppert, C.J. Donahue, A. Moore, and A. Ashkenazi. 1996. J. Biol. Chem. 271:12687.
6. Wiley, S.R., K. Schooley, P.J. Smolak, W.S. Din, C. Huang, J.K. Nicholl, G.R. Sutherland, T.D. Smith, C. Rauch, C.A. Smith, R.G. Goodwin. 1995. Immunity 3:673.
7. Schneider, P., M. Thome, K. Burnes, J. Bodmer, K. Hofmann, T. Kataoka, N. Holler, and J. Tschopp. 1997. Immunity 7:831.
8. Mongkolsapaya, J., J.M. Grimes, N. Chen, X. Xu, D.I. Stuart, E.Y. Jones, and G.R. Screaton. 1999. Nat. Struct. Biol. 6:1048.
9. Sheridan, J.P., S.A. Marsters, R.M. Pitti, A. Gurney, M. Skubatch, D. Baldwin, L. Ramakrishnan, C.L. Gray, K. Baker, W.I. Wood, A.D. Goddard, P. Godowski, and A. Ashkenazi. 1997. Science 277:818.
10. Pan, G., J. Ni, Y. Wei, G. Yu, R. Gentz, and V.M. Dixit. 1997. Science 277:815.
11. Nimmanapalli, R., C.L. Perkins, M. Orlando, E. O'Bryan, D. Nguyen, and K.N. Bhalla. 2001. Cancer Res. 61:759.
12. LaVallee, T.M. X.H. Zhan, M.S. Johnson, C.J. Herstritt, G. Swartz, M.S. Williams, W.A. Hembrough, S.J. Green, and V.S. Pribluda. 2003. Cancer Res. 63:468.
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Product Notes
The Rabbit Anti-Human DR5 (TRAIL-R2) (Catalog #AAA674972) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Human DR5-UNLB reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Rabbit Anti-Human DR5 (TRAIL-R2) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Western Blot. Research Application(s): Induction of apoptosis Flow Cytometry Suggested Dilution: <= 3 ug/10^6 cells Western Blot Suggested Dilution: 1 - 2 ug/mL Induction of Apoptosis Suggested Dilution: 2.5 ug/mL for 12 hours (10-12% in Jurkat cells). Researchers should empirically determine the suitability of the Rabbit Anti-Human DR5 (TRAIL-R2) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rabbit Anti-Human DR5 (TRAIL-R2), Polyclonal Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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