Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine/threonine-protein kinase PLK3 (PLK3) Standard Curve.)
Human Serine/Threonine-Protein Kinase PLK3 (PLK3) ELISA Kit | PLK3 elisa kit
Human Serine/Threonine-Protein Kinase PLK3 (PLK3) ELISA Kit
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine/threonine-protein kinase PLK3 (PLK3) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Serine/threonine-protein kinase PLK3 (PLK3) Standard Curve.)
Background: CNK is a member of the 'polo' family of serine/threonine kinases. Members of this family have been implicated in cell division and are important in regulating the onset of mitosis and M-phase progression.The predicted 607-amino acid protein has an N-terminal catalytic domain, including an ATP-binding site, a central putative nuclear targeting signal, and a presumed C-terminal regulatory domain. The CNK protein shows homology with several other members of the 'polo' family; it is 91% identical to mouse Fnk, 50% identical to human PLK, 48% identical to Drosophila polo, and 38% identical to S. cerevisiae Cdc5. Northern blot analysis detected expression of a 2.5-kb CNK transcript in a limited number of human tissues, with placenta containing a moderate level and ovary, lung, and peripheral blood leukocytes containing low levels.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the highly conserved polo-like kinase family of serine/threonine kinases. Members of this family are characterized by an amino-terminal kinase domain and a carboxy-terminal bipartite polo box domain that functions as a substrate-binding motif and a cellular localization signal. Polo-like kinases are important regulators of cell cycle progression. This gene has also been implicated in stress responses and double-strand break repair. In human cell lines, this protein is reported to associate with centrosomes in a microtubule-dependent manner, and during mitosis, the protein becomes localized to the mitotic apparatus. Expression of a kinase-defective mutant results in abnormal cell morphology caused by changes in microtubule dynamics and mitotic arrest followed by apoptosis. [provided by RefSeq, Sep 2015]
Uniprot Description
PLK3: a kinase of the PLK family. Phosphorylated and activated following DNA damage or mitotic spindle disruption. Interacts with Chk2 and the interaction is enhanced upon DNA damage. Apparent substrates include Chk2 and p53. Plays an important role in regulating microtubule dynamics and centrosomal function. Deregulated expression of Plk3 results in cell cycle arrest and apoptosis.
Protein type: Nucleolus; EC 2.7.11.21; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; Other group; PLK family
Chromosomal Location of Human Ortholog: 1p34.1
Cellular Component: centrosome; Golgi stack; cell soma; cytoplasm; dendrite; nucleolus; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; p53 binding; ATP binding
Biological Process: apoptosis; mitotic cell cycle checkpoint; negative regulation of transcription from RNA polymerase II promoter; endomitotic cell cycle; response to osmotic stress; protein amino acid phosphorylation; regulation of cytokinesis; cytoplasmic microtubule organization and biogenesis; response to reactive oxygen species; response to radiation; protein kinase B signaling cascade; G2/M transition of mitotic cell cycle; regulation of cell division; response to DNA damage stimulus; negative regulation of apoptosis; G1/S transition of mitotic cell cycle
Research Articles on PLK3
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Product Notes
The Human PLK3 plk3 (Catalog #AAA9714871) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714871 ELISA Kit recognizes Human PLK3. It is sometimes possible for the material contained within the vial of "Serine/Threonine-Protein Kinase PLK3 (PLK3), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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