Rabbit GPR210 Polyclonal Antibody | anti-GPR210 antibody

GPR210 polyclonal antibody

Cat. Num. AAA3014900

• Gene Names: PLK3; CNK; FNK; PRK
• Reactivity: Human, Rat, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Synonyms: GPR210; Polyclonal Antibody; GPR210 polyclonal antibody; Serine/threonine-protein kinase PLK3; Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase; Polo-like kinase 3; PLK-3; Proliferation-related kinase; PLK3; CNK; FNK; PRK; anti-GPR210 antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: PLK3 polyclonal antibody detects endogenous levels of PLK3 protein.
• Purity/Purification: The protein was purified from E Coli and the purity is >95% (by SDS-PAGE)
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.4
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-GPR210 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500~1000
• Immunogen: Synthetic peptide, corresponding to Human PLK3
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -25 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of PLK3 polyclonal antibody at 1:500 dilutionLaneA: PC12 whole cell lysateLaneB: CT-26 whole cell lysateLaneC: A549 whole cell lysateLaneD: H1792 whole cell lysate)

Related Product Information for anti-GPR210 antibody

At least 4 distinct polo-like kinases exist in mammalian cells: PLK1, PLK2, PLK3 and PLK4/SAK. Like the other PLK family members, PLK3 contains an amino-terminal catalytic domain and a conserved carboxy-terminal domain termed the Polo box. PLK3, also called proliferation-related kinase (Prk), was originally described as a fibroblast growth factor (FGF)-inducible kinase (Fnk) and identified as an immediate-early response gene responsive to FGF-1 and other mitogens. PLK3 is a cytokine-inducible serine/threonine kinase whose protein expression is cell cycle regulated. Though its expression is found primarily in G1 phase of the cell cycle, PLK3 is detected in G0 and in late telophase prior to cytokinesis. Like the other PLK family members, PLK3 functions mainly as a regulator of the cell cycle. Specifically, PLK3 is required for entry into S phase and is a critical regulator of G1 events, as indicated by RNAi-induced PLK3-depleted cells. PLK3 is also involved in the regulation of DNA damage response via phosphorylation of p53 on Ser20. PLK3 may act as a tumor suppressor as Plk3-deficient mice develop spontaneous tumors in various organs. Unlike PLK1, PLK3 expression is down regulated in cancers including lung, head and neck, and colon.

NCBI and Uniprot Product Information

• NCBI GI #: 41872374
• NCBI GeneID: 1263
• NCBI Accession #: NP_004064.2
• NCBI GenBank Nucleotide #: NM_004073.2
• UniProt Accession #: Q9H4B4; Q15767; Q5JR99; Q96CV1
• Molecular Weight: 71,629 Da
• NCBI Official Full Name: serine/threonine-protein kinase PLK3
• NCBI Official Synonym Full Names: polo-like kinase 3
• NCBI Official Symbol: PLK3
• NCBI Official Synonym Symbols: CNK; FNK; PRK
• NCBI Protein Information: serine/threonine-protein kinase PLK3; PLK-3; FGF-inducible kinase; cytokine-inducible kinase; proliferation-related kinase; cytokine-inducible serine/threonine-protein kinase
• UniProt Protein Name: Serine/threonine-protein kinase PLK3
• UniProt Gene Name: PLK3
• UniProt Synonym Gene Names: CNK; FNK; PRK; PLK-3
• UniProt Entry Name: PLK3_HUMAN

NCBI Description

Cytokine-inducible kinase is a putative serine/threonine kinase. CNK contains both a catalytic domain and a putative regulatory domain. It may play a role in regulation of cell cycle progression and tumorigenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

PLK3: a kinase of the PLK family. Phosphorylated and activated following DNA damage or mitotic spindle disruption. Interacts with Chk2 and the interaction is enhanced upon DNA damage. Apparent substrates include Chk2 and p53. Plays an important role in regulating microtubule dynamics and centrosomal function. Deregulated expression of Plk3 results in cell cycle arrest and apoptosis.

Protein type: Nucleolus; EC 2.7.11.21; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Kinase, protein; Other group; PLK family

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: centrosome; Golgi stack; cell soma; cytoplasm; dendrite; nucleolus; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; p53 binding; ATP binding

Biological Process: apoptosis; mitotic cell cycle checkpoint; negative regulation of transcription from RNA polymerase II promoter; endomitotic cell cycle; response to osmotic stress; protein amino acid phosphorylation; regulation of cytokinesis; cytoplasmic microtubule organization and biogenesis; response to reactive oxygen species; response to radiation; protein kinase B signaling cascade; G2/M transition of mitotic cell cycle; regulation of cell division; response to DNA damage stimulus; negative regulation of apoptosis; G1/S transition of mitotic cell cycle