Human Septin 12 (SEPT12) ELISA Kit | SEPT12 elisa kit

Human Septin 12 (SEPT12) ELISA Kit

Cat. Num. AAA2705940

• Gene Names: SEPT12; SPGF10
• Reactivity: Human
• Synonyms: Septin 12 (SEPT12); Human Septin 12 (SEPT12) ELISA Kit; SEPT12 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Septin 12 (SEPT12).; No significant cross-reactivity or interference between Septin 12 (SEPT12) and analogues was observed.
• Sequence Length: 312

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.064ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Septin 12 (SEPT12) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Septin 12 (SEPT12) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEPT12 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Septin 12 (SEPT12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Septin 12 (SEPT12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Septin 12 (SEPT12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Septin 12 (SEPT12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 23242699
• NCBI GeneID: 124404
• UniProt Accession #: Q8IYM1; Q0P6B0; Q1PBH0; Q96LL0
• Molecular Weight: 35,191 Da
• NCBI Official Full Name: Septin 12
• NCBI Official Synonym Full Names: septin 12
• NCBI Official Symbol: SEPT12
• NCBI Official Synonym Symbols: SPGF10
• NCBI Protein Information: septin-12
• UniProt Protein Name: Septin-12
• UniProt Gene Name: SEPT12

NCBI Description

This gene encodes a guanine-nucleotide binding protein and member of the septin family of cytoskeletal GTPases. Septins play important roles in cytokinesis, exocytosis, embryonic development, and membrane dynamics. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

Filament-forming cytoskeletal GTPase (). Involved in spermatogenesis. Involved in the morphogenesis of sperm heads and the elongation of sperm tails probably implicating the association with alpha- and beta-tubulins (PubMed:24213608). Forms a filamentous structure with SEPT7, SEPT6, SEPT2 and probably SEPT4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (PubMed:25588830). May play a role in cytokinesis (Potential).