Rabbit 4E-BP1 Polyclonal Antibody | anti-4E-BP1 antibody

4E-BP1 (Phospho-Thr45) Antibody

Cat. Num. AAA9502950

• Gene Names: EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Synonyms: 4E-BP1; Polyclonal Antibody; 4E-BP1 (Phospho-Thr45) Antibody; 4EBP1; EIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Insulin-stimulated EIF-4E binding protein PHAS-I; P/OKCL.6; PHAS-1; PHAS-I; anti-4E-BP1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: 4E-BP1 (Phospho-Thr45) Antibody detects endogenous levels of 4E-BP1 only when phosphorylated at Thr45.
• Purity/Purification: Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 118

• Applicable Applications for anti-4E-BP1 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: WB: 1:500~1:1000; IHC: 1:50~1:100; ELISA: 1:20000
• Target Modification: Phospho
• Modification Sites: H:T46; M:T45; R:T45
• Immunogen: The antiserum was produced against synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Thr45.
• Immunogen Range: 13-62
• Preparation and Storage: Stable at -20 degree C for at least 1 year.

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr45) Antibody. The picture on the right is blocked with the phospho peptide.)

Western Blot (WB)

(Western blot analysis of lysates from MDA-MB-435 cells treated with EGF 200ng/ml 5', using 4E-BP1 (Phospho-Thr45) Antibody. The lane on the right is blocked with the phospho peptide.)

NCBI and Uniprot Product Information

• NCBI GI #: 4758258
• NCBI GeneID: 1978
• NCBI Accession #: NP_004086.1
• NCBI GenBank Nucleotide #: NM_004095.3
• UniProt Accession #: Q13541; Q6IBN3; B2R502; D3DSW8
• Molecular Weight: 12 kDa
• NCBI Official Full Name: eukaryotic translation initiation factor 4E-binding protein 1
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4E binding protein 1
• NCBI Official Symbol: EIF4EBP1
• NCBI Official Synonym Symbols: BP-1; 4EBP1; 4E-BP1; PHAS-I
• NCBI Protein Information: eukaryotic translation initiation factor 4E-binding protein 1
• UniProt Protein Name: Eukaryotic translation initiation factor 4E-binding protein 1
• UniProt Gene Name: EIF4EBP1
• UniProt Synonym Gene Names: 4E-BP1; eIF4E-binding protein 1; PHAS-I

NCBI Description

This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]

Uniprot Description

Repressor of translation initiation that regulates EIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. In contrast, hyperphosphorylated form dissociates from EIF4E, allowing interaction between EIF4G1/EIF4G3 and EIF4E, leading to initiation of translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.