Human Cytoglobin (CYGB) ELISA Kit | CYGB elisa kit

Human Cytoglobin (CYGB) ELISA Kit

Cat. Num. AAA2704443

• Gene Names: CYGB; HGB; STAP
• Reactivity: Human
• Synonyms: Cytoglobin (CYGB); Human Cytoglobin (CYGB) ELISA Kit; HGB; STAP; Stellate Cell Activation-Associated Protein; Histoglobin; CYGB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cytoglobin (CYGB).; No significant cross-reactivity or interference between Cytoglobin (CYGB) and analogues was observed.
• Sequence Length: 190

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.064ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytoglobin (CYGB) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytoglobin (CYGB) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYGB elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytoglobin (CYGB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytoglobin (CYGB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytoglobin (CYGB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytoglobin (CYGB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 19549331
• NCBI GeneID: 114757
• NCBI Accession #: NP_599030.1
• NCBI GenBank Nucleotide #: NM_134268.4
• UniProt Accession #: Q8WWM9; Q541Y7; Q8N2X5
• Molecular Weight: 21,405 Da
• NCBI Official Full Name: cytoglobin
• NCBI Official Synonym Full Names: cytoglobin
• NCBI Official Symbol: CYGB
• NCBI Official Synonym Symbols: HGB; STAP
• NCBI Protein Information: cytoglobin
• UniProt Protein Name: Cytoglobin
• Protein Family: Cytoglobin
• UniProt Gene Name: CYGB
• UniProt Synonym Gene Names: STAP; HGb

NCBI Description

This gene encodes a globin protein found in vertebrate cells. The encoded protein is described as a hexacoordinate hemoglobin which binds ligand differently from the pentacoordinate hemoglobins involved in oxygen transport, and may be involved in protection during oxidative stress. This gene is located on chromosome 17 in the same region as a retinal gene which is mutated in progressive rod-cone degeneration, but in the opposite orientation. [provided by RefSeq, Jan 2012]

Uniprot Description

May have a protective function during conditions of oxidative stress. May be involved in intracellular oxygen storage or transfer.