Human Septin 5 (SEPT5) ELISA Kit | SEPT5 elisa kit

Human Septin 5 (SEPT5) ELISA Kit

Cat. Num. AAA2020725

• Gene Names: SEPT5; H5; CDCREL; PNUTL1; CDCREL1; CDCREL-1; HCDCREL-1
• Reactivity: Human
• Synonyms: Septin 5 (SEPT5); Human Septin 5 (SEPT5) ELISA Kit; CDCREL; CDCREL-1; CDCREL1; H5; PNUTL1; Peanut-Like 1; Cell division control-related protein 1; SEPT5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Septin 5 (SEPT5).; No significant cross-reactivity or interference between Septin 5 (SEPT5) and analogues was observed.
• Sequence Length: 369

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.126ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Septin 5 (SEPT5) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Septin 5 (SEPT5) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEPT5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Septin 5 (SEPT5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Septin 5 (SEPT5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Septin 5 (SEPT5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Septin 5 (SEPT5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SEPT5 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 19264128
• NCBI GeneID: 5413
• UniProt Accession #: Q99719; O15251; Q96MY5
• Molecular Weight: 39,332 Da
• NCBI Official Full Name: Septin 5
• NCBI Official Synonym Full Names: septin 5
• NCBI Official Symbol: SEPT5
• NCBI Official Synonym Symbols: H5; CDCREL; PNUTL1; CDCREL1; CDCREL-1; HCDCREL-1
• NCBI Protein Information: septin-5
• UniProt Protein Name: Septin-5
• UniProt Gene Name: SEPT5
• UniProt Synonym Gene Names: PNUTL1; CDCrel-1
• UniProt Entry Name: SEPT5_HUMAN

NCBI Description

This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. [provided by RefSeq, Dec 2010]

Uniprot Description

SEPT5: Filament-forming cytoskeletal GTPase. May play a role in cytokinesis (Potential). May play a role in platelet secretion. Belongs to the septin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Vesicle; Hydrolase; Cell cycle regulation

Chromosomal Location of Human Ortholog: 22q11.21

Cellular Component: synaptic vesicle; cytoskeleton; plasma membrane; terminal button; cell cortex

Molecular Function: GTPase activity; protein binding; GTP binding; structural molecule activity

Biological Process: regulation of exocytosis; metabolic process; cytokinesis; synaptic vesicle targeting